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Applied and Environmental Microbiology, August 2005, p. 4233-4240, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4233-4240.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Study of Adhesion and Survival of Lactobacilli and Bifidobacteria on Table Olives with the Aim of Formulating a New Probiotic Food

Paola Lavermicocca,1* Francesca Valerio,1 Stella Lisa Lonigro,1 Maria De Angelis,1 Lorenzo Morelli,2 Maria Luisa Callegari,3 Carlo G. Rizzello,4 and Angelo Visconti1

Institute of Sciences of Food Production, National Research Council, Bari,1 Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Piacenza,2 Centro Ricerche Biotecnologiche, Università Cattolica del Sacro Cuore, Cremona,3 Dipartimento di Protezione delle Piante e Microbiologia Applicata, Facoltà di Agraria, Università degli Studi di Bari, Bari, Italy4

Received 1 October 2004/ Accepted 3 March 2005

With the aim of developing new functional foods, a traditional product, the table olive, was used as a vehicle for incorporating probiotic bacterial species. Survival on table olives of Lactobacillus rhamnosus (three strains), Lactobacillus paracasei (two strains), Bifidobacterium bifidum (one strain), and Bifidobacterium longum (one strain) at room temperature was investigated. The results obtained using a selected olive sample demonstrated that bifidobacteria and one strain of L. rhamnosus (Lactobacillus GG) showed a good survival rate, with a recovery of about 106 CFU g–1 after 30 days. The Lactobacillus GG population remained unvaried until the end of the experiment, while a slight decline (to about 105 CFU g–1) was observed for bifidobacteria. High viability, with more than 107 CFU g–1, was observed throughout the 3-month experiment for L. paracasei IMPC2.1. This strain, selected for its potential probiotic characteristics and for its lengthy survival on olives, was used to validate table olives as a carrier for transporting bacterial cells into the human gastrointestinal tract. L. paracasei IMPC2.1 was recovered from fecal samples in four out of five volunteers fed 10 to 15 olives per day carrying about 109 to 1010 viable cells for 10 days.

* Corresponding author. Mailing address: Institute of Sciences of Food Production (ISPA), National Research Council (CNR), Via Amendola, 122/O, 70126 Bari, Italy. Phone: 39 0805929356. Fax: 39 0805929374. E-mail: paola.lavermicocca{at}ispa.cnr.it.

Applied and Environmental Microbiology, August 2005, p. 4233-4240, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4233-4240.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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