This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mouwen, D. J. M.
Right arrow Articles by Prieto, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mouwen, D. J. M.
Right arrow Articles by Prieto, M.
Agricola
Right arrow Articles by Mouwen, D. J. M.
Right arrow Articles by Prieto, M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 2005, p. 4318-4324, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4318-4324.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Discrimination of Enterobacterial Repetitive Intergenic Consensus PCR Types of Campylobacter coli and Campylobacter jejuni by Fourier Transform Infrared Spectroscopy

D. J. M. Mouwen,1 M. J. B. M. Weijtens,2 R. Capita,1 C. Alonso-Calleja,1 and M. Prieto1*

Department of Food Hygiene and Technology, University of León, 24071 León, Spain,1 Department of Food and Veterinary Affairs, Ministry of Agriculture, Nature and Food Quality, P.O. Box 20401, 2500 EK The Hague, The Netherlands2

Received 21 October 2004/ Accepted 22 February 2005

Fourier transform infrared spectroscopy (FT-IR) has been used together with pattern recognition methodology to study isolates belonging to the species Campylobacter coli and Campylobacter jejuni and to compare FT-IR typing schemes with established genomic profiles based on enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). Seventeen isolates were cultivated under standardized conditions for 2, 3, and 4 days to study variability and improve reproducibility. ERIC-PCR profiles and FT-IR spectra were obtained from strains belonging to the species Campylobacter coli and C. jejuni, normalized, and explored by hierarchical clustering and stepwise discriminant analysis. Strains could be differentiated by using mainly the first-derivative FT-IR spectral range, 1,200 to 900 cm–1 (described as the carbohydrate region). The reproducibility index varied depending on the ages of the cultures and on the spectral ranges investigated. Classification obtained by FT-IR spectroscopy provided valuable taxonomic information and was mostly in agreement with data from the genotypic method, ERIC-PCR. The classification functions obtained from the discriminant analysis allowed the identification of 98.72% of isolates from the validation set. FT-IR can serve as a valuable tool in the classification, identification, and typing of thermophilic Campylobacter isolates, and a number of types can be differentiated by means of FT-IR spectroscopy.

* Corresponding author. Mailing address: Department of Food Hygiene and Technology, University of León, E-24071 León, Spain. Phone: 34 987 291 283. Fax: 34 987 291 284. E-mail: dhtmpm{at}unileon.es.

Applied and Environmental Microbiology, August 2005, p. 4318-4324, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4318-4324.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»