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Applied and Environmental Microbiology, August 2005, p. 4510-4515, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4510-4515.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Concurrent Quantitation of Total Campylobacter and Total Ciprofloxacin-Resistant Campylobacter Loads in Rinses from Retail Raw Chicken Carcasses from 2001 to 2003 by Direct Plating at 42°C

Ramakrishna Nannapaneni,* Robert Story, Keith C. Wiggins, and Michael G. Johnson

Department of Food Science, and Center for Food Safety & Quality—Institute of Food Science & Engineering, University of Arkansas, Fayetteville, Arkansas 72704

Received 29 June 2004/ Accepted 3 March 2005

This is the first report on the use of a normally lethal dose of ciprofloxacin in a Campylobacter agar medium to kill all ciprofloxacin-sensitive Campylobacter spp. but allow the selective isolation and quantitation of naturally occurring presumptive ciprofloxacin-resistant Campylobacter CFU in rinses from retail raw chicken carcasses (RTCC). Thermophilic-group total Campylobacter CFU and total ciprofloxacin-resistant Campylobacter CFU (irrespective of species) were concurrently quantified in rinses from RTCC by direct plating of centrifuged pellets from 10 or 50 ml out of 400-ml rinse subsamples concurrently on Campylobacter agar and ciprofloxacin-containing Campylobacter agar at 42°C (detection limit = 0.90 log10 CFU/carcass). For 2001, 2002, and 2003, countable Campylobacter CFU were recovered from 85%, 96%, and 57% of RTCC, while countable ciprofloxacin-resistant Campylobacter CFU were recovered from 60%, 59%, and 17.5% of RTCC, respectively. Total Campylobacter CFU loads in RTCC rinses ranged from 0.90 to 4.52, 0.90 to 4.58, and 0.90 to 4.48 log10 CFU/carcass in 2001, 2002, and 2003, respectively. Total ciprofloxacin-resistant Campylobacter CFU loads in RTCC rinses ranged from 0.90 to 4.06, 0.90 to 3.95, and 0.90 to 3.04 log10 CFU/carcass in 2001, 2002, and 2003, respectively. Overall, total Campylobacter loads of 0.90 to 2.0, 2 to 3, 3 to 4, 4 to 5 log10 CFU/carcass, respectively, were recovered from 16%, 32%, 26%, and 5% of RTCC tested over the 2-year sampling period. For the same period, total ciprofloxacin-resistant Campylobacter loads of 0.90 to 2.0, 2 to 3, 3 to 4, and 4 to 5 log10 CFU/carcass, respectively, were recovered from 24%, 11%, 7%, and 0.2% of RTCC tested. There was a steady decline in total Campylobacter and total ciprofloxacin-resistant Campylobacter loads in RTCC rinses from 2001/2002 to 2003.

* Corresponding author. Mailing address: Department of Food Science, and Center for Food Safety & Quality—Institute of Food Science & Engineering, University of Arkansas, Fayetteville, AR 72704. Phone: (479) 575-4206. Fax: (479) 575-3941. E-mail: rkneni{at}uark.edu.

Applied and Environmental Microbiology, August 2005, p. 4510-4515, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4510-4515.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Stern, N. J., Georgsson, F., Lowman, R., Bisaillon, J.-R., Reiersen, J., Callicott, K. A., Geirsdottir, M., Hrolfsdottir, R., Hiett, K. L., the Campy-on-Ice Consortium, (2007). Frequency and Enumeration of Campylobacter Species from Processed Broiler Carcasses by Weep and Rinse Samples. Poult. Sci. 86: 394-399 [Abstract] [Full Text]  


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