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Applied and Environmental Microbiology, August 2005, p. 4585-4591, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4585-4591.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Involvement of a Branched-Chain Aminotransferase in Production of Volatile Sulfur Compounds in Yarrowia lipolytica

Daniela Cernat Bondar,1 Jean-Marie Beckerich,2 and Pascal Bonnarme1*

Institut National de la Recherche Agronomique, Unité Mixte de Recherches Génie et Microbiologie des Procédés Alimentaires,1 Unité Mixte de Recherches de Microbiologie et Génétique Moléculaire, CBAI, 78850 Thiverval-Grignon, France2

Received 6 December 2004/ Accepted 25 February 2005

The enzymatic degradation of L-methionine and the subsequent formation of volatile sulfur compounds (VSCs) are essential for the development of the typical flavor in cheese. In the yeast Yarrowia lipolytica, the degradation of L-methionine was accompanied by the formation of the transamination product 4-methylthio-2-oxobutyric acid. A branched-chain aminotransferase gene (YlBCA1) of Y. lipolytica was amplified, and the L-methionine-degrading activity and the aminotransferase activity were measured in a genetically modified strain and compared to those of the parental strain. Our work shows that L-methionine degradation via transamination is involved in formation of VSCs in Y. lipolytica.

* Corresponding author. Mailing address: INRA, Unité Mixte de Recherches Génie et Microbiologie des Procédés Alimentaires, CBAI, 78850 Thiverval-Grignon, France. Phone: 33 1 30 81 53 88. Fax: 33 1 30 81 55 97. E-mail: bonnarme{at}grignon.inra.fr.

Applied and Environmental Microbiology, August 2005, p. 4585-4591, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4585-4591.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mansour, S., Beckerich, J. M., Bonnarme, P. (2008). Lactate and Amino Acid Catabolism in the Cheese-Ripening Yeast Yarrowia lipolytica. Appl. Environ. Microbiol. 74: 6505-6512 [Abstract] [Full Text]  
  • Cholet, O., Henaut, A., Hebert, A., Bonnarme, P. (2008). Transcriptional Analysis of L-Methionine Catabolism in the Cheese-Ripening Yeast Yarrowia lipolytica in Relation to Volatile Sulfur Compound Biosynthesis. Appl. Environ. Microbiol. 74: 3356-3367 [Abstract] [Full Text]  
  • Cholet, O., Henaut, A., Casaregola, S., Bonnarme, P. (2007). Gene Expression and Biochemical Analysis of Cheese-Ripening Yeasts: Focus on Catabolism of L-Methionine, Lactate, and Lactose. Appl. Environ. Microbiol. 73: 2561-2570 [Abstract] [Full Text]  
  • Kagkli, D.-M., Bonnarme, P., Neuveglise, C., Cogan, T. M., Casaregola, S. (2006). L-Methionine Degradation Pathway in Kluyveromyces lactis: Identification and Functional Analysis of the Genes Encoding L-Methionine Aminotransferase.. Appl. Environ. Microbiol. 72: 3330-3335 [Abstract] [Full Text]  


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