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Applied and Environmental Microbiology, August 2005, p. 4875-4878, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4875-4878.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Presence and Characterization of a Mosaic Genomic Island Which Distinguishes Sorbitol-Fermenting Enterohemorrhagic Escherichia coli O157:H^– from E. coli O157:H7

Institut für Hygiene, Universitätsklinikum Münster, Robert-Koch Str. 41, D-48149 Münster, Germany,¹ Institut für Molekulare Infektionsbiologie, Universität Würzburg, Röntgenring 11, D-97070 Würzburg, Germany²

Received 5 August 2004/ Accepted 21 February 2005

A mosaic genomic island comprising Shigella resistance locus (SRL) sequences flanked by segments of Escherichia coli O157:H7 strain EDL933 O islands 43, 81, and 82 was identified in sorbitol-fermenting (SF) enterohemorrhagic Escherichia coli (EHEC) O157:H^– strain 493/89. This mosaic island is absent from strain EDL933. PCR targeting the SRL-related sequence is a useful tool to distinguish SF EHEC O157:H^– from EHEC O157:H7.

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