This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Suenaga, H.
Right arrow Articles by Kanagawa, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Suenaga, H.
Right arrow Articles by Kanagawa, T.
Agricola
Right arrow Articles by Suenaga, H.
Right arrow Articles by Kanagawa, T.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, August 2005, p. 4879-4884, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4879-4884.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Novel Approach to Quantitative Detection of Specific rRNA in a Microbial Community, Using Catalytic DNA

Hikaru Suenaga,* Rui Liu, Yuko Shiramasa, and Takahiro Kanagawa

Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8566, Japan

Received 5 November 2004/ Accepted 21 February 2005

We developed a novel method for the quantitative detection of the 16S rRNA of a specific bacterial species in the microbial community by using deoxyribozyme (DNAzyme), which possesses the catalytic function to cleave RNA in a sequence-specific manner. A mixture of heterogeneous 16S rRNA containing the target 16S rRNA was incubated with a species-specific DNAzyme. The cleaved target 16S rRNA was separated from the intact 16S rRNA by electrophoresis, and then their amounts were compared for the quantitative detection of target 16S rRNA. This method was used to determine the abundance of the 16S rRNA of a filamentous bacterium, Sphaerotilus natans, in activated sludge, which is a microbial mixture used in wastewater treatment systems. The result indicated that this DNAzyme-based approach would be applicable to actual microbial communities.

* Corresponding author. Mailing address: Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8566, Japan. Phone: 81-29-861-6026. Fax: 81-29-861-6400. E-mail: suenaga-hikaru{at}aist.go.jp.

Applied and Environmental Microbiology, August 2005, p. 4879-4884, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4879-4884.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Silverman, S. K. (2005). In vitro selection, characterization, and application of deoxyribozymes that cleave RNA. Nucleic Acids Res 33: 6151-6163 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»