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Applied and Environmental Microbiology, August 2005, p. 4888-4892, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4888-4892.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Multilocus Simple Sequence Repeat Markers for Differentiating Strains and Evaluating Genetic Diversity of Xylella fastidiosa

Hong Lin,1* Edwin L. Civerolo,1 Rong Hu,2 Samuel Barros,2 Marta Francis,3 and M. Andrew Walker2

Crop Diseases, Pests & Genetics, USDA-ARS, Parlier, California 93548,1 Department of Viticulture and Enology, University of California, Davis, California 95616,2 Department of Plant Pathology, University of California, Davis, California 956163

Received 8 November 2004/ Accepted 22 February 2005

A genome-wide search was performed to identify simple sequence repeat (SSR) loci among the available sequence databases from four strains of Xylella fastidiosa (strains causing Pierce's disease, citrus variegated chlorosis, almond leaf scorch, and oleander leaf scorch). Thirty-four SSR loci were selected for SSR primer design and were validated in PCR experiments. These multilocus SSR primers, distributed across the X. fastidiosa genome, clearly differentiated and clustered X. fastidiosa strains collected from grape, almond, citrus, and oleander. They are well suited for differentiating strains and studying X. fastidiosa epidemiology and population genetics.

* Corresponding author. Mailing address: 9611 S. Riverbend Avenue, Parlier, CA 93648. Phone: (559) 596-2933. Fax: (559) 596-2921. E-mail: hlin{at}fresno.ars.usda.gov.

Applied and Environmental Microbiology, August 2005, p. 4888-4892, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4888-4892.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Randall, J. J., Goldberg, N. P., Kemp, J. D., Radionenko, M., French, J. M., Olsen, M. W., Hanson, S. F. (2009). Genetic Analysis of a Novel Xylella fastidiosa Subspecies Found in the Southwestern United States. Appl. Environ. Microbiol. 75: 5631-5638 [Abstract] [Full Text]  
  • Almeida, R. P. P., Nascimento, F. E., Chau, J., Prado, S. S., Tsai, C.-W., Lopes, S. A., Lopes, J. R. S. (2008). Genetic Structure and Biology of Xylella fastidiosa Strains Causing Disease in Citrus and Coffee in Brazil. Appl. Environ. Microbiol. 74: 3690-3701 [Abstract] [Full Text]  


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