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Applied and Environmental Microbiology, August 2005, p. 4925-4929, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4925-4929.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Genetic Analysis of Two Bile Salt Hydrolase Activities in Lactobacillus acidophilus NCFM

Olivia McAuliffe,1,{dagger} Raul J. Cano,2 and Todd R. Klaenhammer1*

Department of Food Science, Southeast Dairy Foods Research Center, North Carolina State University, Raleigh, North Carolina 27695,1 Environmental Biotechnology Institute, California Polytechnic State University, San Luis Obispo, California 934072

Received 17 November 2004/ Accepted 21 February 2005

Two genes, bshA and bshB, encoding bile salt hydrolase enzymes (EC 3.5.1.24) were identified in the genome sequence of Lactobacillus acidophilus NCFM. Targeted inactivation of these genes via chromosomal insertion of an integration vector demonstrated different substrate specificities for these two enzymes.

* Corresponding author. Mailing address: Dept. of Food Science, North Carolina State University, Raleigh, NC 27695. Phone: (919) 515-2971. Fax: (919) 515-7124. E-mail: trk{at}unity.ncsu.edu.

{dagger} Present address: Dairy Products Research Center, Teagasc, Moorepark, Fermoy, Co. Cork, Ireland.

Applied and Environmental Microbiology, August 2005, p. 4925-4929, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4925-4929.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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