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Applied and Environmental Microbiology, August 2005, p. 4930-4934, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4930-4934.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Distribution of the ermG Gene among Bacterial Isolates from Porcine Intestinal Contents

Yanping Wang,1 Gui-Rong Wang,2 Nadja B. Shoemaker,2* Terence R. Whitehead,3 and Abigail A. Salyers2

New York University School of Medicine, Skirball Institute of Biomolecular Medicine, New York, New York 10016,1 Department of Microbiology, University of Illinois, Urbana, Illinois 61801,2 Fermentation Biotechnology Research Unit, National Center for Agricultural Utilization Research, USDA, Peoria, Illinois 616043

Received 1 April 2004/ Accepted 28 February 2005

The ermG gene was first found in the soil bacterium Bacillus sphaericus. More recently, it was found in several human intestinal Bacteroides species. We report here the first finding of ermG genes in gram-positive bacteria isolated from porcine feces and from under-barn manure pits used to store porcine wastes. The porcine ermG sequences were identical to the sequence of the B. sphaericus ermG gene except that six of the seven ermG-containing strains contained an insertion sequence element insertion in the C-terminal end of the gene. The porcine ermG genes were found in three different gram-positive genera, an indication that it is possible that the gene is being spread by horizontal gene transfer. A segment of a Bacteroides conjugative transposon that carries an ermG gene cross-hybridized with DNA from six of the seven porcine isolates, but the restriction patterns in the porcine strains were different from that of the Bacteroides conjugative transposon.

* Corresponding author. Mailing address: Department of Microbiology, University of Illinois, B103 CLSL, 601 S. Goodwin Ave., Urbana, IL 61801. Phone: (217) 333-2061. Fax: (217) 244-8485. E-mail: nadjasho{at}life.uiuc.edu.

Applied and Environmental Microbiology, August 2005, p. 4930-4934, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4930-4934.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Susanna, K. A., den Hengst, C. D., Hamoen, L. W., Kuipers, O. P. (2006). Expression of Transcription Activator ComK of Bacillus subtilis in the Heterologous Host Lactococcus lactis Leads to a Genome-Wide Repression Pattern: a Case Study of Horizontal Gene Transfer. Appl. Environ. Microbiol. 72: 404-411 [Abstract] [Full Text]  


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