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Applied and Environmental Microbiology, August 2005, p. 4935-4937, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4935-4937.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Identification of Genes Involved in Cytochrome c Biogenesis in Shewanella oneidensis, Using a Modified mariner Transposon

R. Bouhenni, A. Gehrke, and D. Saffarini*

Department of Biological Sciences, University of Wisconsin—Milwaukee, Milwaukee, Wisconsin

Received 4 October 2004/ Accepted 11 March 2005

A modified mariner transposon, miniHimar RB1, was generated to mutagenize cells of the metal-reducing bacterium Shewanella oneidensis. The use of this transposon led to the isolation of stable mutants and allowed rapid identification of disrupted genes. Fifty-eight mutants, including BG104 and BG148 with transposon insertions in the cytochrome c maturation genes ccmC and ccmF1, respectively, were analyzed. Both mutants were deficient in anaerobic respiration and cytochrome c production.

* Corresponding author. Mailing address: Department of Biological Sciences, University of Wisconsin—Milwaukee, 3209 N. Maryland Ave., Milwaukee, WI 53211. Phone: (414) 229-2964. Fax: (414) 229-3926. E-mail: daads{at}uwm.edu.

Applied and Environmental Microbiology, August 2005, p. 4935-4937, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4935-4937.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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