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 Previous Article

Applied and Environmental Microbiology, August 2005, p. 4945-4949, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4945-4949.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Specificity of a Bacteroides thetaiotaomicron Marker for Human Feces

C. Andrew Carson,1* Jessica M. Christiansen,1 Helen Yampara-Iquise,1 Verel W. Benson,2 Claire Baffaut,2 Jerri V. Davis,6 Robert R. Broz,3 William B. Kurtz,5 Wendi M. Rogers,2 and William H. Fales4

Department of Veterinary Pathobiology,1 Department of Agricultural Economics—Food and Agricultural Policy Research Institute,2 Agricultural Extension—Food Science and Nutrition,3 Veterinary Medical Diagnostic Laboratory,4 Department of Forestry, University of Missouri, Columbia, Missouri 65211,5 U.S. Geological Survey, Rolla, Missouri 654016

Received 14 September 2004/ Accepted 15 March 2005

A bacterial primer set, known to produce a 542-bp amplicon specific for Bacteroides thetaiotaomicron, generated this product in PCR with 1 ng of extracted DNA from 92% of 25 human fecal samples, 100% of 20 sewage samples, and 16% of 31 dog fecal samples. The marker was not detected in 1 ng of fecal DNA from 61 cows, 35 horses, 44 pigs, 24 chickens, 29 turkeys, and 17 geese.

* Corresponding author. Mailing address: 201 Connaway Hall, University of Missouri, Columbia, MO 65211. Phone: (573) 884-7640. Fax: (573) 884-0521. E-mail: carsonc{at}missouri.edu.

Applied and Environmental Microbiology, August 2005, p. 4945-4949, Vol. 71, No. 8
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.8.4945-4949.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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