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Applied and Environmental Microbiology, September 2005, p. 5532-5543, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5532-5543.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Complexation of Uranium by Cells and S-Layer Sheets of Bacillus sphaericus JG-A12

Mohamed L. Merroun,1* Johannes Raff,1 André Rossberg,1 Christoph Hennig,1 Tobias Reich,2 and Sonja Selenska-Pobell1

Institute of Radiochemistry, Forschungszentrum Rossendorf, Dresden,1 Institute of Nuclear Chemistry, Johannes Gutenberg Universität Mainz, Mainz, Germany2

Received 23 December 2004/ Accepted 13 April 2005

Bacillus sphaericus JG-A12 is a natural isolate recovered from a uranium mining waste pile near the town of Johanngeorgenstadt in Saxony, Germany. The cells of this strain are enveloped by a highly ordered crystalline proteinaceous surface layer (S-layer) possessing an ability to bind uranium and other heavy metals. Purified and recrystallized S-layer proteins were shown to be phosphorylated by phosphoprotein-specific staining, inductive coupled plasma mass spectrometry analysis, and a colorimetric method. We used extended X-ray absorption fine-structure (EXAFS) spectroscopy to determine the structural parameters of the uranium complexes formed by purified and recrystallized S-layer sheets of B. sphaericus JG-A12. In addition, we investigated the complexation of uranium by the vegetative bacterial cells. The EXAFS analysis demonstrated that in all samples studied, the U(VI) is coordinated to carboxyl groups in a bidentate fashion with an average distance between the U atom and the C atom of 2.88 ± 0.02 Å and to phosphate groups in a monodentate fashion with an average distance between the U atom and the P atom of 3.62 ± 0.02 Å. Transmission electron microscopy showed that the uranium accumulated by the cells of this strain is located in dense deposits at the cell surface.

* Corresponding author. Mailing address: Institute of Radiochemistry, Forschungszentrum Rossendorf, P.O. Box 510119, D-01314 Dresden, Germany. Phone: 49-351-2602946. Fax: 49-351-2603553. E-mail: m.merroun{at}fz-rossendorf.de.

Applied and Environmental Microbiology, September 2005, p. 5532-5543, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5532-5543.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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