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Applied and Environmental Microbiology, September 2005, p. 5582-5586, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5582-5586.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

S434F in NrdE Generates the Thermosensitive Phenotype of Corynebacterium ammoniagenes CH31 and Enhances Thermolability by Increasing the Surface Hydrophobicity of the NrdE(Ts) Protein

Hesham M. Elhariry,1,{dagger} Jochen Meens,1,{ddagger} Matthias Stehr,2 and Georg Auling1*

Institut für Mikrobiologie, Universität Hannover, Schneiderberg 50, D-30167 Hannover, Germany,1 GBF—Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-38124 Braunschweig, Germany2

Received 25 November 2004/ Accepted 31 March 2005

The thermosensitive phenotype of strain CH31, a derivative of Corynebacterium ammoniagenes ATCC 6872, was allocated by cloning, sequencing, and genetic complementation to a single C->T exchange in the nrdE (nucleotide reduction) gene at nucleotide 1301. Protein modeling indicates the impaired surface hydrophobicity of NrdE(Ts) due to the S434F transition.

* Corresponding author. Mailing address: Institut für Mikrobiologie der Universität, Schneiderberg 50, D-30167 Hannover, Germany. Phone: 49/511-762 5241. Fax: 49/511-762 5287. E-mail: auling{at}ifmb.uni-hannover.de.

{dagger} Present address: Department of Food Science, Faculty of Agriculture, Ain Shams University, Cairo, Egypt.

{ddagger} Present address: Zentrum für Infektionsmedizin-Institut für Mikrobiologie, Stiftung Tierärztliche Hochschule Hannover, Bischofsholer Damm 15, D-30173 Hannover, Germany.

Applied and Environmental Microbiology, September 2005, p. 5582-5586, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5582-5586.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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Copyright © 2005 by the American Society for Microbiology. All rights reserved.