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Applied and Environmental Microbiology, September 2005, p. 5593-5597, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5593-5597.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Identification of DNA Sequences Specific for Vibrio vulnificus Biotype 2 Strains by Suppression Subtractive Hybridization

Chung-Te Lee,1 Carmen Amaro,3 Eva Sanjuán,3 and Lien-I Hor1,2*

Institute of Basic Medical Sciences,1 Department of Microbiology and Immunology, College of Medicine, National Cheng-Kung University, Tainan 701, Taiwan, Republic of China,2 Departamento de Microbiología y Ecología, Facultad de Biología, Universidad de Valencia, 46100 Valencia, Spain3

Received 20 October 2004/ Accepted 4 April 2005

Vibrio vulnificus can be divided into three biotypes, and only biotype 2, which is further divided into serovars, contains eel-virulent strains. We compared the genomic DNA of a biotype 2 serovar E isolate (tester) with the genomic DNAs of three biotype 1 strains by suppression subtractive hybridization and then tested the distribution of the tester-specific DNA sequences in a wide collection of bacterial strains. In this way we identified three plasmid-borne DNA sequences that were specific for biotype 2 strains irrespective of the serovar and three chromosomal DNA sequences that were specific for serovar E biotype 2 strains. These sequences have potential for use in the diagnosis of eel vibriosis caused by V. vulnificus and in the detection of biotype 2 serovar E strains.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, College of Medicine, National Cheng-Kung University, Tainan 701, Taiwan, Republic of China. Phone: 886-6-2353535, ext. 5635. Fax: 886-6-2082705. E-mail: h061453{at}mail.ncku.edu.tw.

Applied and Environmental Microbiology, September 2005, p. 5593-5597, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5593-5597.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Roig, F. J., Amaro, C. (2009). Plasmid diversity in Vibrio vulnificus biotypes. Microbiology 155: 489-497 [Abstract] [Full Text]  
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  • Valiente, E., Jimenez, N., Merino, S., Tomas, J. M., Amaro, C. (2008). Vibrio vulnificus Biotype 2 Serovar E gne but Not galE Is Essential for Lipopolysaccharide Biosynthesis and Virulence. Infect. Immun. 76: 1628-1638 [Abstract] [Full Text]  
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