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Applied and Environmental Microbiology, September 2005, p. 5604-5606, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5604-5606.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Molecular Characterization of Clostridium tetani Strains by Pulsed-Field Gel Electrophoresis and Colony PCR

Lucile Plourde-Owobi, Delphine Seguin, Marie-Anne Baudin, Catherine Moste, and Bachra Rokbi*

Aventis Pasteur, Marcy L'Etoile, France

Received 30 August 2004/ Accepted 29 March 2005

Pulsed-field gel electrophoresis and PCR were applied for the first time to the molecular characterization of Clostridium tetani. Among five strains tested, one (CN1339) turned out to contain a mixture of two genetically different clones and two (D11 and G761) to contain bacteria differing by the presence or absence of the 74-kb plasmid harboring the tetX gene.

* Corresponding author. Mailing address: Research Department, Aventis Pasteur SA, av. Marcel Mérieux, Marcy L'Etoile 69280, France. Phone: 33(4) 37 37 36 05. Fax: 33(4) 37 37 31 89. E-mail: bachra.rokbi{at}aventis.com.

Applied and Environmental Microbiology, September 2005, p. 5604-5606, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5604-5606.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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