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Applied and Environmental Microbiology, September 2005, p. 5610-5613, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5610-5613.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Biosynthesis of Natural Flavanones in Saccharomyces cerevisiae

Yajun Yan, Abhijeet Kohli, and Mattheos A. G. Koffas*

Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, New York 14260

Received 28 February 2005/ Accepted 14 April 2005

A four-step flavanone biosynthetic pathway was constructed and introduced into Saccharomyces cerevisiae. The recombinant yeast strain was fed with phenylpropanoid acids and produced the flavanones naringenin and pinocembrin 62 and 22 times more efficiently compared to previously reported recombinant prokaryotic strains. Microbial biosynthesis of the flavanone eriodictyol was also achieved.

* Corresponding author. Mailing address: Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, 904 Furnas Hall, Buffalo, NY 14260. Phone: (716) 645-2911, ext. 2221. Fax: (716) 645-3822. E-mail: mkoffas{at}buffalo.edu.

Applied and Environmental Microbiology, September 2005, p. 5610-5613, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5610-5613.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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