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Applied and Environmental Microbiology, September 2005, p. 5614-5618, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5614-5618.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Improved System for Construction and Analysis of Single-Copy ß-Galactosidase Operon Fusions in Yersinia enterocolitica

Michelle E. Maxson and Andrew J. Darwin*

Department of Microbiology, New York University School of Medicine, New York, New York 10016

Received 22 January 2005/ Accepted 21 April 2005

We report a significantly improved system for studying single-copy lacZ operon fusions in Yersinia enterocolitica: a simple procedure for the stable integration of lacZ operon fusions into the ara locus and a strain with a deletion mutation that abolishes the low level of endogenous ß-galactosidase activity.

* Corresponding author. Mailing address: Department of Microbiology MSB 228, New York University School of Medicine, 550 First Avenue, New York, NY 10016. Phone: (212) 263-3223. Fax: (212) 263-8276. E-mail: darwia01{at}med.nyu.edu.

Applied and Environmental Microbiology, September 2005, p. 5614-5618, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5614-5618.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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