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Applied and Environmental Microbiology, September 2005, p. 5614-5618, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5614-5618.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Improved System for Construction and Analysis of Single-Copy ß-Galactosidase Operon Fusions in Yersinia enterocolitica

Michelle E. Maxson and Andrew J. Darwin*

Department of Microbiology, New York University School of Medicine, New York, New York 10016

Received 22 January 2005/ Accepted 21 April 2005

We report a significantly improved system for studying single-copy lacZ operon fusions in Yersinia enterocolitica: a simple procedure for the stable integration of lacZ operon fusions into the ara locus and a strain with a deletion mutation that abolishes the low level of endogenous ß-galactosidase activity.

* Corresponding author. Mailing address: Department of Microbiology MSB 228, New York University School of Medicine, 550 First Avenue, New York, NY 10016. Phone: (212) 263-3223. Fax: (212) 263-8276. E-mail: darwia01{at}med.nyu.edu.

Applied and Environmental Microbiology, September 2005, p. 5614-5618, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5614-5618.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Axler-DiPerte, G. L., Hinchliffe, S. J., Wren, B. W., Darwin, A. J. (2009). YtxR Acts as an Overriding Transcriptional Off Switch for the Yersinia enterocolitica Ysc-Yop Type 3 Secretion System. J. Bacteriol. 191: 514-524 [Abstract] [Full Text]  
  • Lawrenz, M. B., Miller, V. L. (2007). Comparative Analysis of the Regulation of rovA from the Pathogenic Yersiniae. J. Bacteriol. 189: 5963-5975 [Abstract] [Full Text]  
  • Axler-DiPerte, G. L., Miller, V. L., Darwin, A. J. (2006). YtxR, a Conserved LysR-Like Regulator That Induces Expression of Genes Encoding a Putative ADP-Ribosyltransferase Toxin Homologue in Yersinia enterocolitica. J. Bacteriol. 188: 8033-8043 [Abstract] [Full Text]  
  • Maxson, M. E., Darwin, A. J. (2006). Multiple promoters control expression of the Yersinia enterocolitica phage-shock-protein A (pspA) operon.. Microbiology 152: 1001-1010 [Abstract] [Full Text]  


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