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Applied and Environmental Microbiology, September 2005, p. 5646-5649, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5646-5649.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Extracellular Protease of Pseudomonas fluorescens CHA0, a Biocontrol Factor with Activity against the Root-Knot Nematode Meloidogyne incognita

Imran Ali Siddiqui,1 Dieter Haas,2 and Stephan Heeb2*

Soil Biology and Ecology Laboratory, Department of Botany, University of Karachi, Karachi-75270, Pakistan,1 Département de Microbiologie Fondamentale, Université de Lausanne, CH-1015 Lausanne, Switzerland2

Received 7 October 2004/ Accepted 7 April 2005

In Pseudomonas fluorescens CHA0, mutation of the GacA-controlled aprA gene (encoding the major extracellular protease) or the gacA regulatory gene resulted in reduced biocontrol activity against the root-knot nematode Meloidogyne incognita during tomato and soybean infection. Culture supernatants of strain CHA0 inhibited egg hatching and induced mortality of M. incognita juveniles more strongly than did supernatants of aprA and gacA mutants, suggesting that AprA protease contributes to biocontrol.

* Corresponding author. Present address: Institute of Infection, Immunity and Inflammation, Centre for Biomolecular Sciences, University of Nottingham, Nottingham NG7 2RD, United Kingdom. Phone: 44 (0115) 951 50 89. Fax: 44 (0115) 846 79 51. E-mail: Stephan.Heeb{at}nottingham.ac.uk.

Applied and Environmental Microbiology, September 2005, p. 5646-5649, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5646-5649.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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