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Applied and Environmental Microbiology, September 2005, p. 5650-5653, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5650-5653.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Characterization of a Multicopper Oxidase Gene from Staphylococcus aureus

S. Sitthisak, K. Howieson, C. Amezola, and R. K. Jayaswal*

Department of Biological Sciences, Illinois State University, Normal, Illinois 61790-4120

Received 17 December 2004/ Accepted 11 April 2005

A multicopper oxidase gene from Staphylococcus aureus was cloned and overexpressed. Purified recombinant multicopper oxidase oxidized the substrate 3,3'-dimethoxybenzidine in the presence of copper. Disruption of mco showed copper sensitivity and H2O2 resistance, suggesting roles for mco in copper homeostasis and oxidative stress response. Northern blot analysis showed copper-induced mco transcription.

* Corresponding author. Mailing address: Microbiology Group, Department of Biological Sciences, Illinois State University, Normal, IL 61790-4120. Phone: (309) 438-5128. Fax: (309) 438-3722. E-mail: drjay{at}ilstu.edu.

Applied and Environmental Microbiology, September 2005, p. 5650-5653, Vol. 71, No. 9
0099-2240/05/$08.00+0     doi:10.1128/AEM.71.9.5650-5653.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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