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Applied and Environmental Microbiology, January 2006, p. 233-238, Vol. 72, No. 1
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.1.233-238.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Production and Characterization of a Thermostable Alcohol Dehydrogenase That Belongs to the Aldo-Keto Reductase Superfamily

Ronnie Machielsen,* Agustinus R. Uria,{dagger} Servé W. M. Kengen, and John van der Oost

Laboratory of Microbiology, Wageningen University, Wageningen, The Netherlands

Received 16 September 2005/ Accepted 27 October 2005

The gene encoding a novel alcohol dehydrogenase that belongs to the aldo-keto reductase superfamily has been identified in the hyperthermophilic archaeon Pyrococcus furiosus. The gene, referred to as adhD, was functionally expressed in Escherichia coli and subsequently purified to homogeneity. The enzyme has a monomeric conformation with a molecular mass of 32 kDa. The catalytic activity of the enzyme increases up to 100°C, and a half-life value of 130 min at this temperature indicates its high thermostability. AdhD exhibits a broad substrate specificity with, in general, a preference for the reduction of ketones (pH optimum, 6.1) and the oxidation of secondary alcohols (pH optimum, 8.8). Maximal specific activities were detected with 2,3-butanediol (108.3 U/mg) and diacetyl-acetoin (22.5 U/mg) in the oxidative and reductive reactions, respectively. Gas chromatrography analysis indicated that AdhD produced mainly (S)-2-pentanol (enantiomeric excess, 89%) when 2-pentanone was used as substrate. The physiological role of AdhD is discussed.

* Corresponding author. Mailing address: Laboratory of Microbiology, Hesselink van Suchtelenweg 4, 6703 CT Wageningen, The Netherlands. Phone: 31-317-483748. Fax: 31-317-483829. E-mail: Ronnie.machielsen{at}wur.nl

{dagger} Present address: Laboratory of Marine Biotechnology, Research Center for Marine and Fisheries Product Processing and Biotechnology, Jl. K.S. Tubun Petamburan VI, Central Jakarta 10260, Indonesia.

Applied and Environmental Microbiology, January 2006, p. 233-238, Vol. 72, No. 1
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.1.233-238.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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