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Applied and Environmental Microbiology, January 2006, p. 346-352, Vol. 72, No. 1
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.1.346-352.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Real-Time PCR for the Detection and Quantification of Geodermatophilaceae from Stone Samples and Identification of New Members of the Genus Blastococcus{dagger}

Oscar Salazar,* Aranzazu Valverde, and Olga Genilloud

Centro de Investigación Básica de España, Merck Research Laboratories, Merck Sharp & Dohme de España S.A., Josefa Valcárcel 38, 28027 Madrid, Spain

Received 27 May 2005/ Accepted 3 October 2005

Real-time PCR (RT-PCR) technology was used for the specific detection and quantification of members of the family Geodermatophilaceae in stone samples. Differences in the nucleotide sequences of the 16S rRNA gene region were used to design a pair of family-specific primers that were used to detect and quantify by RT-PCR DNA from members of this family in stone samples from different geographical origins in Spain. These primers were applied later to identify by PCR-specific amplification new members of the family Geodermatophilaceae isolated from the same stone samples. The diversity and taxonomic position of the wild-type strains identified from ribosomal sequence analysis suggest the presence of a new lineage within the genus Blastococcus.

* Corresponding author. Mailing address: Centro de Investigación Básica de España, Merck Research Laboratories, Merck Sharp and Dohme de España S.A., Josefa Valcárcel 38, E-28027 Madrid, Spain. Phone: 34913210493. Fax: 34913210614. E-mail: oscar_salazar{at}merck.com

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, January 2006, p. 346-352, Vol. 72, No. 1
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.1.346-352.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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