This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van Beilen, J. B.
Right arrow Articles by Witholt, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van Beilen, J. B.
Right arrow Articles by Witholt, B.
Agricola
Right arrow Articles by van Beilen, J. B.
Right arrow Articles by Witholt, B.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, January 2006, p. 59-65, Vol. 72, No. 1
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.1.59-65.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Cytochrome P450 Alkane Hydroxylases of the CYP153 Family Are Common in Alkane-Degrading Eubacteria Lacking Integral Membrane Alkane Hydroxylases{dagger}

Jan B. van Beilen,* Enrico G. Funhoff, Alexander van Loon, Andrea Just, Leo Kaysser, Manuel Bouza, René Holtackers, Martina Röthlisberger, Zhi Li, and Bernard Witholt

Institute of Biotechnology, ETH Hönggerberg, CH-8093 Zürich, Switzerland

Received 1 June 2005/ Accepted 23 September 2005

Several strains that grow on medium-chain-length alkanes and catalyze interesting hydroxylation and epoxidation reactions do not possess integral membrane nonheme iron alkane hydroxylases. Using PCR, we show that most of these strains possess enzymes related to CYP153A1 and CYP153A6, cytochrome P450 enzymes that were characterized as alkane hydroxylases. A vector for the polycistronic coexpression of individual CYP153 genes with a ferredoxin gene and a ferredoxin reductase gene was constructed. Seven of the 11 CYP153 genes tested allowed Pseudomonas putida GPo12 recombinants to grow well on alkanes, providing evidence that the newly cloned P450s are indeed alkane hydroxylases.

* Corresponding author. Mailing address: Institute of Biotechnology, ETH Hönggerberg, CH-8093 Zürich, Switzerland. Phone: 41-1-6333444. Fax: 41-1-6331051. E-mail: vanbeilen{at}biotech.biol.ethz.ch

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, January 2006, p. 59-65, Vol. 72, No. 1
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.1.59-65.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Koch, D. J., Chen, M. M., van Beilen, J. B., Arnold, F. H. (2009). In Vivo Evolution of Butane Oxidation by Terminal Alkane Hydroxylases AlkB and CYP153A6. Appl. Environ. Microbiol. 75: 337-344 [Abstract] [Full Text]  
  • Mattes, T. E., Alexander, A. K., Richardson, P. M., Munk, A. C., Han, C. S., Stothard, P., Coleman, N. V. (2008). The Genome of Polaromonas sp. Strain JS666: Insights into the Evolution of a Hydrocarbon- and Xenobiotic-Degrading Bacterium, and Features of Relevance to Biotechnology. Appl. Environ. Microbiol. 74: 6405-6416 [Abstract] [Full Text]  
  • Dubbels, B. L., Sayavedra-Soto, L. A., Arp, D. J. (2007). Butane monooxygenase of 'Pseudomonas butanovora': purification and biochemical characterization of a terminal-alkane hydroxylating diiron monooxygenase. Microbiology 153: 1808-1816 [Abstract] [Full Text]  
  • Throne-Holst, M., Wentzel, A., Ellingsen, T. E., Kotlar, H.-K., Zotchev, S. B. (2007). Identification of Novel Genes Involved in Long-Chain n-Alkane Degradation by Acinetobacter sp. Strain DSM 17874. Appl. Environ. Microbiol. 73: 3327-3332 [Abstract] [Full Text]  
  • Muller, R. H., Rohwerder, T., Harms, H. (2007). Carbon Conversion Efficiency and Limits of Productive Bacterial Degradation of Methyl tert-Butyl Ether and Related Compounds. Appl. Environ. Microbiol. 73: 1783-1791 [Abstract] [Full Text]  
  • Funhoff, E. G., Bauer, U., Garcia-Rubio, I., Witholt, B., van Beilen, J. B. (2006). CYP153A6, a Soluble P450 Oxygenase Catalyzing Terminal-Alkane Hydroxylation.. J. Bacteriol. 188: 5220-5227 [Abstract] [Full Text]  
  • Sabirova, J. S., Ferrer, M., Regenhardt, D., Timmis, K. N., Golyshin, P. N. (2006). Proteomic Insights into Metabolic Adaptations in Alcanivorax borkumensis Induced by Alkane Utilization. J. Bacteriol. 188: 3763-3773 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»