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Applied and Environmental Microbiology, January 2006, p. 760-768, Vol. 72, No. 1
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.1.760-768.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Involvement of Quinolinate Phosphoribosyl Transferase in Promotion of Potato Growth by a Burkholderia Strain

Keri Wang,{dagger} Kenneth Conn, and George Lazarovits*

Southern Crop Protection and Food Research Center, Agriculture and Agri-Food Canada, 1391 Sandford St., London, Ontario, Canada N5V 4T3

Received 1 June 2005/ Accepted 26 September 2005

Burkholderia sp. strain PsJN stimulates root growth of potato explants compared to uninoculated controls under gnotobiotic conditions. In order to determine the mechanism by which this growth stimulation occurs, we used Tn5 mutagenesis to produce a mutant, H41, which exhibited no growth-promoting activity but was able to colonize potato plants as well as the wild-type strain. The gene associated with the loss of growth promotion in H41 was shown to exhibit 65% identity at the amino acid level to the nadC gene encoding quinolinate phosphoribosyltransferase (QAPRTase) in Ralstonia solanacearum. Complementation of H41 with QAPRTase restored growth promotion of potato explants by this mutant. Expression of the gene identified in Escherichia coli yielded a protein with QAPRTase activities that catalyzed the de novo formation of nicotinic acid mononucleotide (NaMN). Two other genes involved in the same enzymatic pathway, nadA and nadB, were physically linked to nadC. The nadA gene was cotranscribed with nadC as an operon in wild-type strain PsJN, while the nadB gene was located downstream of the nadA-nadC operon. Growth promotion by H41 was fully restored by addition of NaMN to the tissue culture medium. These data suggested that QAPRTase may play a role in the signal pathway for promotion of plant growth by PsJN.

* Corresponding author. Mailing address: Southern Crop Protection and Food Research Center, Agriculture and Agri-Food Canada, 1391 Sandford St., London, ON, Canada N5V 4T3. Phone: (519) 457-1470, ext. 293. Fax: (519) 457-3997. E-mail: lazarovitsg{at}agr.gc.ca

{dagger} Present address: Plant Biology Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401.

Applied and Environmental Microbiology, January 2006, p. 760-768, Vol. 72, No. 1
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.1.760-768.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Schippers, J. H.M., Nunes-Nesi, A., Apetrei, R., Hille, J., Fernie, A. R., Dijkwel, P. P. (2008). The Arabidopsis onset of leaf death5 Mutation of Quinolinate Synthase Affects Nicotinamide Adenine Dinucleotide Biosynthesis and Causes Early Ageing. Plant Cell 20: 2909-2925 [Abstract] [Full Text]  


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