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Applied and Environmental Microbiology, January 2006, p. 927-931, Vol. 72, No. 1
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.1.927-931.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Immobilization of Cells with Surface-Displayed Chitin-Binding Domain

Jen-You Wang and Yun-Peng Chao*

Department of Chemical Engineering, Feng Chia University, 100 Wenhwa Road, Taichung, Taiwan

Received 30 March 2005/ Accepted 19 September 2005

To explore chitin-binding domain (ChBD)-based cell immobilization, a tripartite gene fusion consisting of an in-frame fusion of ChBD to lpp and ompA was constructed and expressed in Escherichia coli. ChBD-displayed cells exhibited highly specific and stable binding to chitin within a wide range of pHs (5 to 8) and temperatures (15 to 37°C). These results illustrate the promising use of this approach for engineering applications.

* Corresponding author. Mailing address: Department of Chemical Engineering, Feng Chia University, 100 Wenhwa Road, Taichung, Taiwan. Phone: 886 2451 7250 3677. Fax: 886 4 2451 0890. E-mail: ypchao{at}fcu.edu.tw

Applied and Environmental Microbiology, January 2006, p. 927-931, Vol. 72, No. 1
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.1.927-931.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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