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Applied and Environmental Microbiology, January 2006, p. 963-967, Vol. 72, No. 1
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.1.963-967.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Expression of the ubiE Gene of Geobacillus stearothermophilus V in Escherichia coli K-12 Mediates the Evolution of Selenium Compounds into the Headspace of Selenite- and Selenate-Amended Cultures

J. W. Swearingen Jr.,3,{dagger} D. E. Fuentes,1,{dagger} M. A. Araya,1,{ddagger} M. F. Plishker,3 C. P. Saavedra,2 T. G. Chasteen,3 and C. C. Vásquez1*

Departamento de Ciencias Biológicas, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, Chile,1 Departamento de Biología, Facultad de Ciencias de la Salud, Universidad Andrés Bello, Santiago, Chile,2 Department of Chemistry, Sam Houston State University, Huntsville, Texas3

Received 26 July 2005/ Accepted 10 October 2005

The ubiE gene of Geobacillus stearothermophilus V, with its own promoter, was cloned and introduced into Escherichia coli. The cloned gene complemented the ubiE gene deficiency of E. coli AN70. In addition, the expression of this gene in E. coli JM109 resulted in the evolution of volatile selenium compounds when these cells were grown in selenite- or selenate-amended media. These compounds were dimethyl selenide and dimethyl diselenide.

* Corresponding author. Mailing address: Laboratorio de Microbiología Molecular, Departamento de Ciencias Biológicas, Facultad de Química y Biología, Universidad de Santiago de Chile, Casilla 40, Correo 33, Santiago, Chile. Phone: (56) 2 681 0357. Fax: (56) 2 681 2108. E-mail: cvasquez{at}usach.cl

{dagger} J.W.S. and D.E.F. contributed equally to this work.

{ddagger} Present address: Roche Diagnostics Chile, Av. Quilín, 3750 Santiago, Chile.

Applied and Environmental Microbiology, January 2006, p. 963-967, Vol. 72, No. 1
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.1.963-967.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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