This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Manninen, T. J. K.
Right arrow Articles by Saris, P. E. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Manninen, T. J. K.
Right arrow Articles by Saris, P. E. J.
Agricola
Right arrow Articles by Manninen, T. J. K.
Right arrow Articles by Saris, P. E. J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, October 2006, p. 6539-6543, Vol. 72, No. 10
0099-2240/06/$08.00+0     doi:10.1128/AEM.02977-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Alteration of the Canine Small-Intestinal Lactic Acid Bacterium Microbiota by Feeding of Potential Probiotics

Titta J. K. Manninen,1 Minna L. Rinkinen,2 Shea S. Beasley,1 and Per E. J. Saris1*

Department of Applied Chemistry and Microbiology,1 Department of Clinical Veterinary Science, University of Helsinki, Helsinki, Finland2

Received 16 December 2005/ Accepted 22 June 2006

Five potentially probiotic canine fecal lactic acid bacterium (LAB) strains, Lactobacillus fermentum LAB8, Lactobacillus salivarius LAB9, Weissella confusa LAB10, Lactobacillus rhamnosus LAB11, and Lactobacillus mucosae LAB12, were fed to five permanently fistulated beagles for 7 days. The survival of the strains and their potential effects on the indigenous intestinal LAB microbiota were monitored for 17 days. Denaturing gradient gel electrophoresis (DGGE) demonstrated that the five fed LAB strains survived in the upper gastrointestinal tract and modified the dominant preexisting indigenous jejunal LAB microbiota of the dogs. When the LAB supplementation was ceased, DGGE analysis of jejunal chyme showed that all the fed LAB strains were undetectable after 7 days. However, the diversity of the intestinal indigenous microbiota of the dogs, as characterized from jejunal chyme plated on Lactobacillus selective medium without acetic acid, was reduced and did not return to the original level during the study period. In all but one dog, an indigenous Lactobacillus acidophilus strain emerged as the dominant LAB strain. In conclusion, strains LAB8 to LAB12 have potential as probiotic strains for dogs as they survive in and dominate the jejunal LAB microbiota during feeding and have the ability to modify the intestinal microbiota.

* Corresponding author. Mailing address: Department of Applied Chemistry and Microbiology, Viikinkaari 9, FI-00014 University of Helsinki, Finland. Phone: 358-9-19159369. Fax: 385-9-19159322. E-mail: per.saris{at}helsinki.fi.

Applied and Environmental Microbiology, October 2006, p. 6539-6543, Vol. 72, No. 10
0099-2240/06/$08.00+0     doi:10.1128/AEM.02977-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Millette, M., Cornut, G., Dupont, C., Shareck, F., Archambault, D., Lacroix, M. (2008). Capacity of Human Nisin- and Pediocin-Producing Lactic Acid Bacteria To Reduce Intestinal Colonization by Vancomycin-Resistant Enterococci. Appl. Environ. Microbiol. 74: 1997-2003 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»