This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Baker, J. R.
Right arrow Articles by Pritchard, D. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baker, J. R.
Right arrow Articles by Pritchard, D. G.
Agricola
Right arrow Articles by Baker, J. R.
Right arrow Articles by Pritchard, D. G.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, October 2006, p. 6825-6828, Vol. 72, No. 10
0099-2240/06/$08.00+0     doi:10.1128/AEM.00829-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Endopeptidase and Glycosidase Activities of the Bacteriophage B30 Lysin

John R. Baker, Chengbao Liu, Shengli Dong, and David G. Pritchard*

Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama

Received 7 April 2006/ Accepted 16 July 2006

Synthetic peptides corresponding to portions of group B streptococcal peptidoglycan were used to show that the endopeptidase activity of bacteriophage B30 lysin cleaves between D-Ala in the stem peptide and L-Ala in the cross bridge and that the minimal peptide sequence cleaved is DL-{gamma}-Glu-Lys-D-Ala-Ala-Ala. The only glycosidase activity present is that of N-acetyl-ß-D-muramidase.

* Corresponding author. Mailing address: Department of Biochemistry & Molecular Genetics, MCLM 552, University of Alabama at Birmingham, 1530 3rd Ave. S, Birmingham, AL 35294-0005. Phone: (205) 934-6023. Fax: (205) 934-6022. E-mail: davidp1{at}uab.edu.

Applied and Environmental Microbiology, October 2006, p. 6825-6828, Vol. 72, No. 10
0099-2240/06/$08.00+0     doi:10.1128/AEM.00829-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pritchard, D. G., Dong, S., Kirk, M. C., Cartee, R. T., Baker, J. R. (2007). LambdaSa1 and LambdaSa2 Prophage Lysins of Streptococcus agalactiae. Appl. Environ. Microbiol. 73: 7150-7154 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»