This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Penton, C. R.
Right arrow Articles by Tiedje, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Penton, C. R.
Right arrow Articles by Tiedje, J. M.
Agricola
Right arrow Articles by Penton, C. R.
Right arrow Articles by Tiedje, J. M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, October 2006, p. 6829-6832, Vol. 72, No. 10
0099-2240/06/$08.00+0     doi:10.1128/AEM.01254-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Molecular Evidence for the Broad Distribution of Anaerobic Ammonium-Oxidizing Bacteria in Freshwater and Marine Sediments

C. Ryan Penton,1* Allan H. Devol,2 and James M. Tiedje1

Center for Microbial Ecology, Michigan State University, East Lansing, Michigan 48824,1 School of Oceanography, University of Washington, Seattle, Washington 981952

Received 31 May 2006/ Accepted 31 July 2006

Previously available primer sets for detecting anaerobic ammonium-oxidizing (anammox) bacteria are inefficient, resulting in a very limited database of such sequences, which limits knowledge of their ecology. To overcome this limitation, we designed a new primer set that was 100% specific in the recovery of ~700-bp 16S rRNA gene sequences with >96% homology to the "Candidatus Scalindua" group of anammox bacteria, and we detected this group at all sites studied, including a variety of freshwater and marine sediments and permafrost soil. A second primer set was designed that exhibited greater efficiency than previous primers in recovering full-length (1,380-bp) sequences related to "Ca. Scalindua," "Candidatus Brocadia," and "Candidatus Kuenenia." This study provides evidence for the widespread distribution of anammox bacteria in that it detected closely related anammox 16S rRNA gene sequences in 11 geographically and biogeochemically diverse freshwater and marine sediments.

* Corresponding author. Mailing address: Michigan State University, 540 Plant and Soil Sciences Bldg., East Lansing, MI 48824. Phone: (517) 355-0271, ext. 284. Fax: (517) 353-2917. E-mail: pentonch{at}msu.edu.

Applied and Environmental Microbiology, October 2006, p. 6829-6832, Vol. 72, No. 10
0099-2240/06/$08.00+0     doi:10.1128/AEM.01254-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Dong, L. F., Smith, C. J., Papaspyrou, S., Stott, A., Osborn, A. M., Nedwell, D. B. (2009). Changes in Benthic Denitrification, Nitrate Ammonification, and Anammox Process Rates and Nitrate and Nitrite Reductase Gene Abundances along an Estuarine Nutrient Gradient (the Colne Estuary, United Kingdom). Appl. Environ. Microbiol. 75: 3171-3179 [Abstract] [Full Text]  
  • Mulvaney, M. J., Cummins, K. A., Wood, C. W., Wood, B. H., Tyler, P. J. (2008). Ammonia Emissions from Field-Simulated Cattle Defecation and Urination. J. Environ. Qual. 37: 2022-2027 [Abstract] [Full Text]  
  • van Niftrik, L., Geerts, W. J. C., van Donselaar, E. G., Humbel, B. M., Webb, R. I., Fuerst, J. A., Verkleij, A. J., Jetten, M. S. M., Strous, M. (2008). Linking Ultrastructure and Function in Four Genera of Anaerobic Ammonium-Oxidizing Bacteria: Cell Plan, Glycogen Storage, and Localization of Cytochrome c Proteins. J. Bacteriol. 190: 708-717 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»