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Applied and Environmental Microbiology, November 2006, p. 6894-6901, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.00928-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Inactivation of Adhesion and Invasion of Food-Borne Listeria monocytogenes by Bacteriocin-Producing Bifidobacterium Strains of Human Origin{triangledown}

Olivier Moroni,1 Ehab Kheadr,1,2 Yvan Boutin,3 Christophe Lacroix,4 and Ismaïl Fliss1*

STELA Dairy Research Center, Nutraceuticals and Functional Foods Institute (INAF), Pavillon Paul Comtois, Université Laval, Québec, Québec, Canada G1K 7P4,1 Department of Dairy Science and Technology, Faculty of Agriculture, University of Alexandria, Alexandria, Egypt,2 TransBiotech, CEGEP Lévis-Lauzon, Lévis, Québec, Canada G6V 9V6,3 Institute of Food Science and Nutrition, Swiss Federal Institute of Technology, ETH Zentrum, LFO F18, CH-8092 Zurich, Switzerland4

Received 19 April 2006/ Accepted 14 August 2006

Three bacteriocin-producing bifidobacterial isolates from newborns were identified as Bifidobacterium thermacidophilum (two strains) and B. thermophilum (one strain). This study was undertaken to evaluate the ability of these strains to compete with food-borne Listeria monocytogenes for adhesion and invasion sites on Caco-2 and HT-29 cells. The bifidobacteria adhered at levels ranging from 4% to 10% of the CFU added, but none of the bifidobacteria were able to invade cells. The abilities of Listeria to adhere to and to invade cells varied widely depending on the strain tested. Three groups of Listeria were identified based on invasiveness: weakly invasive, moderately invasive, and highly invasive strains. One strain from each group was tested in competition with bifidobacteria. B. thermacidophilum RBL70 was the most effective in blocking invasion of Listeria, and the decreases in invasion ranged from 38% to 90%. For all three bifidobacterial strains, contact between the cell monolayer and the bifidobacteria for 1 h before exposure to Listeria increased the degree of inhibition. Finally, visualization of competition for adhesion sites on cells by fluorescent in situ hybridization suggested that the two bacteria tended to adhere in close proximity.

* Corresponding author. Mailing address: Department of Food and Nutrition, Pavillon Paul-Comtois, Laval University, Quebec, PQ, Canada G1K 7P4. Phone: (418) 656-2131, ext. 6825. Fax: (418) 656-3353. E-mail: ismail.fliss{at}aln.ulaval.ca.

{triangledown} Published ahead of print on 25 August 2006.

Applied and Environmental Microbiology, November 2006, p. 6894-6901, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.00928-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ollinger, J., Bowen, B., Wiedmann, M., Boor, K. J., Bergholz, T. M. (2009). Listeria monocytogenes {sigma}B Modulates PrfA-Mediated Virulence Factor Expression. Infect. Immun. 77: 2113-2124 [Abstract] [Full Text]  


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