AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
AEM.00763-06v1
72/11/7022    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sintes, E.
Right arrow Articles by Herndl, G. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sintes, E.
Right arrow Articles by Herndl, G. J.
Agricola
Right arrow Articles by Sintes, E.
Right arrow Articles by Herndl, G. J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2006, p. 7022-7028, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.00763-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Quantifying Substrate Uptake by Individual Cells of Marine Bacterioplankton by Catalyzed Reporter Deposition Fluorescence In Situ Hybridization Combined with Microautoradiography{triangledown}

Eva Sintes* and Gerhard J. Herndl

Department of Biological Oceanography, Royal Netherlands Institute for Sea Research, P.O. Box 59, 1790 AB Den Burg, The Netherlands

Received 2 April 2006/ Accepted 21 August 2006

Catalyzed reporter deposition fluorescence in situ hybridization combined with microautoradiography (MICRO-CARD-FISH) is increasingly being used to obtain qualitative information on substrate uptake by individual members of specific prokaryotic communities. Here we evaluated the potential for using this approach quantitatively by relating the measured silver grain area around cells taking up 3H-labeled leucine to bulk leucine uptake measurements. The increase in the silver grain area over time around leucine-assimilating cells of coastal bacterial assemblages was linear during 4 to 6 h of incubation. By establishing standardized conditions for specific activity levels and concomitantly performing uptake measurements with the bulk community, MICRO-CARD-FISH can be used quantitatively to determine uptake rates on a single-cell level. Therefore, this approach allows comparisons of single-cell activities for bacterial communities obtained from different sites or growing under different ecological conditions.

* Corresponding author. Mailing address: Department of Biological Oceanography, Royal Netherlands Institute for Sea Research (NIOZ), P.O. Box 59, 1790 AB Den Burg, Texel, The Netherlands. Phone: 31-222-369-523. Fax: 31-222-319-674. E-mail: esintes{at}nioz.nl.

{triangledown} Published ahead of print on 1 September 2006.

Applied and Environmental Microbiology, November 2006, p. 7022-7028, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.00763-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2006 by the American Society for Microbiology. All rights reserved.