This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Monnerat, R.
Right arrow Articles by Bravo, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Monnerat, R.
Right arrow Articles by Bravo, A.
Agricola
Right arrow Articles by Monnerat, R.
Right arrow Articles by Bravo, A.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2006, p. 7029-7035, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.01454-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Genetic Variability of Spodoptera frugiperda Smith (Lepidoptera: Noctuidae) Populations from Latin America Is Associated with Variations in Susceptibility to Bacillus thuringiensis Cry Toxins{triangledown}

Rose Monnerat,1 Erica Martins,1 Paulo Queiroz,1 Sergio Ordúz,2 Gabriela Jaramillo,2 Graciela Benintende,3 Jorge Cozzi,3 M. Dolores Real,4 Amparo Martinez-Ramirez,4 Carolina Rausell,4 Jairo Cerón,5 Jorge E. Ibarra,6 M. Cristina Del Rincon-Castro,6 Ana M. Espinoza,7 Luis Meza-Basso,8 Lizbeth Cabrera,9 Jorge Sánchez,9 Mario Soberon,9 and Alejandra Bravo9*

EMBRAPA—Recursos Genéticos e Biotecnologia, W5 Norte Final, CP 02372, CEP-70.770.970 Brasília, Federal District, Brasil,1 Corporación para Investigaciones Biológicas, Carrera 72-A, No. 78B-141, A.A. 7378, and Escuela de Biociencias, Facultad de Ciencias, Universidad Nacional de Colombia, Medellín,2 Instituto de Biotecnología, Universidad Nacional de Colombia, Cd. Universitaria, AA14490, Bogotá, Colombia,5 Instituto de Microbiología y Zoología Agrícola— INTA CC No. 25 (1712) Castelar, Buenos Aires, Argentina,3 Departamento de Genética, Universidad de Valencia, Dr. Moliner 50, Burjassot 46100, Valencia, Spain,4 CINVESTAV IPN, Ap. Postal 629, Irapuato 36500, Guanajuato,6 Instituto de Biotecnología UNAM, Ap. Postal 510-3, Cuernavaca, 62250 Morelos, México,9 Centro de Investigación en Biología Celular y Molecular, Universidad de Costa Rica, Cd. de la Investigación San Pedro, San José, Costa Rica,7 Instituto de Biologia Vegetal y Biotecnologia, Universidad de Talca, Casilla 747, Talca, Chile8

Received 23 June 2006/ Accepted 21 August 2006

Bacillus thuringiensis strains isolated from Latin American soil samples that showed toxicity against three Spodoptera frugiperda populations from different geographical areas (Mexico, Colombia, and Brazil) were characterized on the basis of their insecticidal activity, crystal morphology, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of parasporal crystals, plasmid profiles, and cry gene content. We found that the different S. frugiperda populations display different susceptibilities to the selected B. thuringiensis strains and also to pure preparations of Cry1B, Cry1C, and Cry1D toxins. Binding assays performed with pure toxin demonstrated that the differences in the toxin binding capacities of these insect populations correlated with the observed differences in susceptibility to the three Cry toxins analyzed. Finally, the genetic variability of the three insect populations was analyzed by random amplification of polymorphic DNA-PCR, which showed significant genetic diversity among the three S. frugiperda populations analyzed. The data presented here show that the genetic variability of S. frugiperda populations should be carefully considered in the development of insect pest control strategies, including the deployment of genetically modified maize in different geographical regions.

* Corresponding author. Mailing address: Instituto de Biotecnología UNAM, Ap. Postal 510-3, Cuernavaca, 62250 Morelos, México. Phone: (52-777) 3291635. Fax: (52-777) 3291624. E-mail: bravo{at}ibt.unam.mx.

{triangledown} Published ahead of print on 25 August 2006.

Applied and Environmental Microbiology, November 2006, p. 7029-7035, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.01454-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Reyes-Ramirez, A., Ibarra, J. E. (2008). Plasmid Patterns of Bacillus thuringiensis Type Strains. Appl. Environ. Microbiol. 74: 125-129 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»