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Applied and Environmental Microbiology, November 2006, p. 7238-7245, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.01494-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Genetic Characterization of the Resorcinol Catabolic Pathway in Corynebacterium glutamicum{triangledown}

Yan Huang,1,2 Ke-Xin Zhao,1,2 Xi-Hui Shen,1 Muhammad Tausif Chaudhry,1,2 Cheng-Ying Jiang,1 and Shuang-Jiang Liu1*

State Key Laboratory of Microbial Resource, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080,1 Graduate University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China2

Received 28 June 2006/ Accepted 30 August 2006

Corynebacterium glutamicum grew on resorcinol as a sole source of carbon and energy. By genome-wide data mining, two gene clusters, designated NCgl1110-NCgl1113 and NCgl2950-NCgl2953, were proposed to encode putative proteins involved in resorcinol catabolism. Deletion of the NCgl2950-NCgl2953 gene cluster did not result in any observable phenotype changes. Disruption and complementation of each gene at NCgl1110-NCgl1113, NCgl2951, and NCgl2952 indicated that these genes were involved in resorcinol degradation. Expression of NCgl1112, NCgl1113, and NCgl2951 in Escherichia coli revealed that NCgl1113 and NCgl2951 both coded for hydroxyquinol 1,2-dioxygenases and NCgl1112 coded for maleylacetate reductases. NCgl1111 encoded a putative monooxygenase, but this putative hydroxylase was very different from previously functionally identified hydroxylases. Cloning and expression of NCgl1111 in E. coli revealed that NCgl1111 encoded a resorcinol hydroxylase that needs NADPH as a cofactor. E. coli cells containing Ncgl1111 and Ncgl1113 sequentially converted resorcinol into maleylacetate. NCgl1110 and NCgl2950 both encoded putative TetR family repressors, but only NCgl1110 was transcribed and functional. NCgl2953 encoded a putative transporter, but disruption of this gene did not affect resorcinol degradation by C. glutamicum. The function of NCgl2953 remains unclear.

* Corresponding author. Mailing address: Institute of Microbiology, Chinese Academy of Sciences, ZhongGuanCun, Haidian, Beijing 100080, People's Republic of China. Phone: 86-10-62527118. Fax: 86-10-62652317. E-mail: liusj{at}sun.im.ac.cn.

{triangledown} Published ahead of print on 8 September 2006.

Applied and Environmental Microbiology, November 2006, p. 7238-7245, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.01494-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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