This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ait Barka, E.
Right arrow Articles by Clément, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ait Barka, E.
Right arrow Articles by Clément, C.
Agricola
Right arrow Articles by Ait Barka, E.
Right arrow Articles by Clément, C.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2006, p. 7246-7252, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.01047-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Enhancement of Chilling Resistance of Inoculated Grapevine Plantlets with a Plant Growth-Promoting Rhizobacterium, Burkholderia phytofirmans Strain PsJN{triangledown}

Essaid Ait Barka,1* Jerzy Nowak,2 and Christophe Clément1

Laboratoire de Stress, Défenses et Reproduction des Plantes, Unité de Recherche Vignes et Vins de Champagne, UPRES EA 2069, UFR Sciences, Université de Reims Champagne-Ardenne, 51687 Reims Cédex 2, France,1 Department of Horticulture, Virginia Polytechnic Institute and State University, 0327-301 Saunders Hall, Blacksburg, Virginia 240602

Received 5 May 2006/ Accepted 7 September 2006

In vitro inoculation of Vitis vinifera L. cv. Chardonnay explants with a plant growth-promoting rhizobacterium, Burkholderia phytofirmans strain PsJN, increased grapevine growth and physiological activity at a low temperature. There was a relationship between endophytic bacterial colonization of the grapevine plantlets and their growth at both ambient (26°C) and low (4°C) temperatures and their sensitivities to chilling. The major benefits of bacterization were observed on root growth (11.8- and 10.7-fold increases at 26°C and 4°C, respectively) and plantlet biomass (6- and 2.2-fold increases at 26°C and 4°C, respectively). The inoculation with PsJN also significantly improved plantlet cold tolerance compared to that of the nonbacterized control. In nonchilled plantlets, bacterization enhanced CO2 fixation and O2 evolution 1.3 and 2.2 times, respectively. The nonbacterized controls were more sensitive to exposure to low temperatures than were the bacterized plantlets, as indicated by several measured parameters. Moreover, relative to the noninoculated controls, bacterized plantlets had significantly increased levels of starch, proline, and phenolics. These increases correlated with the enhancement of cold tolerance of the grapevine plantlets. In summary, B. phytofirmans strain PsJN inoculation stimulates grapevine growth and improves its ability to withstand cold stress.

* Corresponding author. Mailing address: Laboratoire de Stress, Défenses et Reproduction des Plantes, Unité de Recherche Vignes et Vins de Champagne, UPRES EA 2069, UFR Sciences, Université de Reims Champagne-Ardenne, 51687 Reims Cédex 2, France. Phone and fax: 33-3-26-91-34-41. E-mail: ea.barka{at}univ-reims.fr.

{triangledown} Published ahead of print on 15 September 2006.

Applied and Environmental Microbiology, November 2006, p. 7246-7252, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.01047-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lebon, G., Wojnarowiez, G., Holzapfel, B., Fontaine, F., Vaillant-Gaveau, N., Clement, C. (2008). Sugars and flowering in the grapevine (Vitis vinifera L.). J Exp Bot 59: 2565-2578 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»