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Applied and Environmental Microbiology, November 2006, p. 7260-7263, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.03028-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Sensitivity of an Immunomagnetic-Separation-Based Test for Detecting Escherichia coli O26 in Bovine Feces{triangledown}

L. M. Hall,1* J. Evans,1 A. W. Smith,1,2 M. C. Pearce,1,2 H. I. Knight,1,2 G. Foster,3 J. C. Low,1 and G. J. Gunn1

Animal Health Group, Scottish Agricultural College, West Mains Road, Edinburgh EH9 3JG,1 Centre for Tropical Veterinary Medicine, University of Edinburgh, Roslin, Midlothian EH25 9RG,2 Veterinary Services, Scottish Agricultural College, Stratherrick Road, Inverness IV2 4JZ, United Kingdom3

Received 23 December 2005/ Accepted 4 September 2006

The sensitivity of a test for cattle shedding Escherichia coli serogroup O26 was estimated using several fecal pats artificially inoculated at a range of concentrations with different E. coli O26 strains. The test involves the enrichment of fecal microflora in buffered peptone water, the selective concentration of E. coli O26 using antibody-coated immunomagnetic-separation beads, the identification of E. coli colonies on Chromocult tryptone bile X-glucuronide agar, and confirmation of the serogroup with E. coli serogroup O26-specific antisera using slide agglutination. The effective dose of E. coli O26 for an 80% test sensitivity (ED80) was 1.0 x 104 CFU g–1 feces (95% confidence interval, 4.7 x 103 to 2.4 x 104). Differences in test sensitivity between different E. coli O26 strains and fecal pats were also observed. Individual estimates of ED80 for each strain and fecal pat combination ranged from 4.2 x 102 to 4.8 x 105 CFU g–1. These results suggest that the test is useful for identifying individuals shedding a large number of E. coli O26 organisms or, if an appropriate number of individuals in a herd are sampled, for identifying affected herds. The study also provides a benchmark estimate of sensitivity that can be used to compare alternative tests for E. coli O26 and a methodological approach that can be applied to tests for other pathogenic members of the Enterobacteriaceae and other sample types.

* Corresponding author. Mailing address: Scottish Agricultural College, Stratherrick Rd., Inverness IV2 4JZ, United Kingdom. Phone: 44 (0) 1463 243030. Fax: 44 (0) 1463 711103. E-mail: malcolm.hall{at}sac.ac.uk.

{triangledown} Published ahead of print on 15 September 2006.

Applied and Environmental Microbiology, November 2006, p. 7260-7263, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.03028-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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