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Applied and Environmental Microbiology, November 2006, p. 7264-7269, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.01477-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Mechanism of Augmentation of Organotin Decomposition by Ferripyochelin: Formation of Hydroxyl Radical and Organotin-Pyochelin-Iron Ternary Complex{triangledown}

Guo-Xin Sun1 and Jian-Jiang Zhong1,2*

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, People's Republic of China,1 Key Laboratory of Microbial Metabolism, Ministry of Education, College of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai 200240, People's Republic of China2

Received 27 June 2006/ Accepted 10 September 2006

Pyochelin (PCH), a kind of siderophore secreted by Pseudomonas aeruginosa, was recently found to have triphenyltin (TPT)-decomposing capacity. In this work, significant augmentation of TPT decomposition by ferripyochelin (FePCH), the chelating compound of PCH with iron, was demonstrated in Tris-HCl buffer (pH 8.0). The generation of hydroxyl radical (HO·) in the presence of FePCH was observed. Inhibition of HO· generation by adding catalase and HO· scavengers (methanol and dimethyl sulfoxide) decreased TPT decomposition, while an increase in HO· formation in the presence of H2O2 enhanced its decomposition. Our findings indicated that HO· generated in the reaction system was responsible for the enhanced TPT decomposition by FePCH versus PCH. The existence of the TPT-pyochelin-iron ternary complex was demonstrated by electron spray ionization-mass spectrometry, tandem mass spectrometry, and 1H nuclear magnetic resonance. On the basis of the above results, HO· produced in the presence of FePCH was deduced to be in close proximity to TPT and has more opportunity to attack the Sn-C bond, which resulted in the enhanced organotin decomposition. The information obtained may have considerable environmental significance.

* Corresponding author. Mailing address: Key Laboratory of Microbial Metabolism, Ministry of Education, College of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dong Chuan Road, Shanghai 200240, People's Republic of China. Phone and fax: 86-21-34204831. E-mail: jjzhong{at}sjtu.edu.cn.

{triangledown} Published ahead of print on 22 September 2006.

Applied and Environmental Microbiology, November 2006, p. 7264-7269, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.01477-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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