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Applied and Environmental Microbiology, November 2006, p. 7359-7364, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.01159-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Genetic Differences between Blight-Causing Erwinia Species with Differing Host Specificities, Identified by Suppression Subtractive Hybridization{triangledown}

Lindsay R. Triplett, Youfu Zhao,{dagger} and George W. Sundin*

Department of Plant Pathology and Center for Microbial Pathogenesis, Michigan State University, East Lansing, Michigan 48824

Received 19 May 2006/ Accepted 23 August 2006

PCR-based subtractive hybridization was used to isolate sequences from Erwinia amylovora strain Ea110, which is pathogenic on apples and pears, that were not present in three closely related strains with differing host specificities: E. amylovora MR1, which is pathogenic only on Rubus spp.; Erwinia pyrifoliae Ep1/96, the causal agent of shoot blight of Asian pears; and Erwinia sp. strain Ejp556, the causal agent of bacterial shoot blight of pear in Japan. In total, six subtractive libraries were constructed and analyzed. Recovered sequences included type III secretion components, hypothetical membrane proteins, and ATP-binding proteins. In addition, we identified an Ea110-specific sequence with homology to a type III secretion apparatus component of the insect endosymbiont Sodalis glossinidius, as well as an Ep1/96-specific sequence with homology to the Yersinia pestis effector protein tyrosine phosphatase YopH.

* Corresponding author. Mailing address: Department of Plant Pathology, Michigan State University, 103 Center for Integrated Plant Systems, East Lansing, MI 48824. Phone: (517) 355-4573. Fax: (517) 353-5598. E-mail: sundin{at}msu.edu.

{triangledown} Published ahead of print on 8 September 2006.

{dagger} Present address: Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801.

Applied and Environmental Microbiology, November 2006, p. 7359-7364, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.01159-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Alavi, S. M., Sanjari, S., Durand, F., Brin, C., Manceau, C., Poussier, S. (2008). Assessment of the Genetic Diversity of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans as a Basis To Identify Putative Pathogenicity Genes and a Type III Secretion System of the SPI-1 Family by Multiple Suppression Subtractive Hybridizations. Appl. Environ. Microbiol. 74: 3295-3301 [Abstract] [Full Text]  


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