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Applied and Environmental Microbiology, November 2006, p. 7368-7372, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.00294-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

In Vivo Insertional Mutagenesis in Corynebacterium pseudotuberculosis: an Efficient Means To Identify DNA Sequences Encoding Exported Proteins{triangledown}

Fernanda A. Dorella,1 Estela M. Estevam,1 Luis G. C. Pacheco,1 Cláudia T. Guimarães,2 Ubiraci G. P. Lana,2 Eliane A. Gomes,2 Michele M. Barsante,3 Sérgio C. Oliveira,3 Roberto Meyer,4 Anderson Miyoshi,1,{dagger} and Vasco Azevedo1*,{dagger}

Laboratório de Genética Celular e Molecular,1 Laboratório de Imunologia de Doenças Infecciosas, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte,3 Núcleo de Biologia Aplicada, Empresa Brasileira de Pesquisa Agropecuária, Sete Lagoas, Minas Gerais,2 Instituto de Ciências da Saúde, Vale do Canela, Salvador, Bahia, Brazil4

Received 2 February 2006/ Accepted 10 August 2006

The reporter transposon-based system TnFuZ was used to identify exported proteins of the animal pathogen Corynebacterium pseudotuberculosis. Thirty-four out of 1,500 mutants had detectable alkaline phosphatase (PhoZ) activity. This activity was from 21 C. pseudotuberculosis loci that code for fimbrial and transport subunits and for hypothetical and unknown-function proteins.

* Corresponding author. Mailing address: Laboratório de Genética Celular e Molecular, Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, CP 486, CEP 30161-970, Belo Horizonte, Minas Gerais, Brazil. Phone and fax: 55 31 34 99 26 10. E-mail: vasco{at}icb.ufmg.br.

{triangledown} Published ahead of print on 1 September 2006.

{dagger} V.A. and A.M. share credit for senior authorship of this work.

Applied and Environmental Microbiology, November 2006, p. 7368-7372, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.00294-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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