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Applied and Environmental Microbiology, November 2006, p. 7406-7409, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.01157-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Isolation and Characterization of a Novel Lipase from a Metagenomic Library of Tidal Flat Sediments: Evidence for a New Family of Bacterial Lipases{triangledown}

Mi-Hwa Lee,1 Choong-Hwan Lee,1 Tae-Kwang Oh,1 Jae Kwang Song,2* and Jung-Hoon Yoon1*

Korea Research Institute of Bioscience and Biotechnology,1 Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea2

Received 19 May 2006/ Accepted 26 August 2006

We cloned lipG, which encoded a lipolytic enzyme, from a Korean tidal flat metagenomic library. LipG was related to six putative lipases previously identified only in bacterial genome sequences. These enzymes comprise a new family. We partially characterized LipG, providing the first experimental data for a member of this family.

* Corresponding author. Mailing address for Jae Kwang Song: Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology, P.O. Box 107, Yuseong, Daejeon 305-600, Korea. Phone: 82 42 860 7643. Fax: 82 42 860 7649. E-mail: ajee{at}krict.re.kr. Mailing address for Jung-Hoon Yoon: Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yuseong, Daejeon 305-333, Korea. Phone: 82 42 860 4276. Fax: 82 42 879 8595. E-mail: jhyoon{at}kribb.re.kr.

{triangledown} Published ahead of print on 1 September 2006.

Applied and Environmental Microbiology, November 2006, p. 7406-7409, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.01157-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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