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Applied and Environmental Microbiology, November 2006, p. 7418-7421, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.01067-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Role of the ptsN Gene Product in Catabolite Repression of the Pseudomonas putida TOL Toluene Degradation Pathway in Chemostat Cultures{triangledown}

Isabel Aranda-Olmedo, Patricia Marín, Juan L. Ramos, and Silvia Marqués*

Consejo Superior de Investigaciones Científicas, Estación Experimental del Zaidín, Dept. of Environmental Protection, C/. Profesor Albareda no. 1, Granada E-18008, Spain

Received 9 May 2005/ Accepted 8 August 2006

The Pseudomonas putida KT2440 TOL upper pathway is repressed under nonlimiting conditions in cells growing in chemostat with succinate as a carbon source. We show that the ptsN gene product IIANtr participates in this repression. Crc, involved in yeast extract-dependent repression in batch cultures, did not influence expression when cells were growing in a chemostat with succinate at maximum rate.


* Corresponding author. Mailing address: Consejo Superior de Investigaciones Científicas, Estación Experimental del Zaidín, Dept. of Environmental Protection, C/. Profesor Albareda no. 1, Granada E-18008, Spain. Phone: 34 958 181600, ext. 285. Fax: 34 958 135740. E-mail: silvia.marques{at}eez.csic.es.

{triangledown} Published ahead of print on 22 September 2006.


Applied and Environmental Microbiology, November 2006, p. 7418-7421, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.01067-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.




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