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Applied and Environmental Microbiology, November 2006, p. 7422-7426, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.01550-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Genetic Characterization of the Phenylacetyl-Coenzyme A Oxygenase from the Aerobic Phenylacetic Acid Degradation Pathway of Escherichia coli{triangledown}

Cristina Fernández,{dagger} Abel Ferrández,{dagger},{ddagger} Baltasar Miñambres,§ Eduardo Díaz, and José L. García*

Department of Molecular Microbiology, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain

Received 5 July 2006/ Accepted 11 September 2006

We show here that the paaABCDE genes of the paa cluster responsible for phenylacetate degradation in Escherichia coli W encode a five-component oxygenase that hydroxylates phenylacetyl-coenzyme A (CoA), the first intermediate of the pathway. The primary structure of the subunits of bacterial phenylacetyl-CoA oxygenases revealed that these enzymes constitute the prototype of a new and distinct group of the large bacterial diiron multicomponent oxygenase family.

* Corresponding author. Mailing address: Department of Molecular Microbiology, Centro de Invetigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Ramiro de Maeztu 9, 28040 Madrid, Spain. Phone: 34-918373112. Fax: 34-915360432. E-mail: JLGARCIA{at}CIB.CSIC.ES.

{triangledown} Published ahead of print on 22 September 2006.

{dagger} C. Fernández and A. Ferrández contributed equally to this work.

{ddagger} Present address: DSM Nutritional Products Ltd., Basel, Switzerland.

§ Present address: Estación Agrícola Experimental, Consejo Superior de Investigaciones Científicas, Grulleros, León, Spain.

Applied and Environmental Microbiology, November 2006, p. 7422-7426, Vol. 72, No. 11
0099-2240/06/$08.00+0     doi:10.1128/AEM.01550-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Law, R. J., Hamlin, J. N. R., Sivro, A., McCorrister, S. J., Cardama, G. A., Cardona, S. T. (2008). A Functional Phenylacetic Acid Catabolic Pathway Is Required for Full Pathogenicity of Burkholderia cenocepacia in the Caenorhabditis elegans Host Model. J. Bacteriol. 190: 7209-7218 [Abstract] [Full Text]  


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