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Applied and Environmental Microbiology, December 2006, p. 7842-7848, Vol. 72, No. 12
0099-2240/06/$08.00+0     doi:10.1128/AEM.01826-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

EhRho1, a RhoA-Like GTPase of Entamoeba histolytica, Is Modified by Clostridial Glucosylating Cytotoxins{triangledown}

Shubhra Majumder,1 Gudula Schmidt,2 Anuradha Lohia,1 and Klaus Aktories2*

Department of Biochemistry, Bose Institute, Kolkata 700054, India,1 Institut für Experimentelle und Klinische Pharmakologie und Toxikologie, Albert-Ludwigs-Universität, D-79104 Freiburg, Germany2

Received 2 August 2006/ Accepted 10 October 2006

Clostridial glucosylating cytotoxins inactivate mammalian Rho GTPases by mono-O glucosylation of a conserved threonine residue located in the switch 1 region of the target protein. Here we report that EhRho1, a RhoA-like GTPase from the protozoan parasite Entamoeba histolytica, is glucosylated by clostridial cytotoxins. Recombinant glutathione S-transferase-EhRho1 and EhRho1 from cell lysate of Entamoeba histolytica were glucosylated by Clostridium difficile toxin B and Clostridium novyi alpha-toxin. In contrast, Clostridium difficile toxin A, which shares the same mammalian protein substrates with toxin B, did not modify EhRho1. Change of threonine 52 of EhRho1 to alanine prevented glucosylation by toxin B from Clostridium difficile and by alpha-toxin from Clostridium novyi, which suggests that the equivalent threonine residues are glucosylated in mammalian and Entamoeba Rho GTPases. Lethal toxin from Clostridium sordellii did not glucosylate EhRho1 but labeled several other substrate proteins in lysates from Entamoeba histolytica in the presence of UDP-[14C]glucose.

* Corresponding author. Mailing address: Institut für Experimentelle und Klinische Pharmakologie und Toxikologie, Albertstraße 25, D-79104 Freiburg, Germany. Phone: 49-761-2035301. Fax: 49-761-2035311. E-mail: Klaus.Aktories{at}pharmakol.uni-freiburg.de.

{triangledown} Published ahead of print on 20 October 2006.

Applied and Environmental Microbiology, December 2006, p. 7842-7848, Vol. 72, No. 12
0099-2240/06/$08.00+0     doi:10.1128/AEM.01826-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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