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Applied and Environmental Microbiology, December 2006, p. 7873-7878, Vol. 72, No. 12
0099-2240/06/$08.00+0     doi:10.1128/AEM.01362-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Engineering of Deinococcus radiodurans R1 for Bioprecipitation of Uranium from Dilute Nuclear Waste{triangledown}

Deepti Appukuttan, Amara Sambasiva Rao, and Shree Kumar Apte*

Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, India

Received 14 June 2006/ Accepted 10 October 2006

Genetic engineering of radiation-resistant organisms to recover radionuclides/heavy metals from radioactive wastes is an attractive proposition. We have constructed a Deinococcus radiodurans strain harboring phoN, a gene encoding a nonspecific acid phosphatase, obtained from a local isolate of Salmonella enterica serovar Typhi. The recombinant strain expressed an ~27-kDa active PhoN protein and efficiently precipitated over 90% of the uranium from a 0.8 mM uranyl nitrate solution in 6 h. The engineered strain retained uranium bioprecipitation ability even after exposure to 6 kGy of 60Co gamma rays. The PhoN-expressing D. radiodurans offers an effective and eco-friendly in situ approach to biorecovery of uranium from dilute nuclear waste.

* Corresponding author. Mailing address: Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India. Phone: 91-22-25595342. Fax: 91-22-25505326. E-mail: sksmbd{at}barc.gov.in.

{triangledown} Published ahead of print on 20 October 2006.

Applied and Environmental Microbiology, December 2006, p. 7873-7878, Vol. 72, No. 12
0099-2240/06/$08.00+0     doi:10.1128/AEM.01362-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Nilgiriwala, K. S., Alahari, A., Rao, A. S., Apte, S. K. (2008). Cloning and Overexpression of Alkaline Phosphatase PhoK from Sphingomonas sp. Strain BSAR-1 for Bioprecipitation of Uranium from Alkaline Solutions. Appl. Environ. Microbiol. 74: 5516-5523 [Abstract] [Full Text]  


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