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Applied and Environmental Microbiology, December 2006, p. 7912-7915, Vol. 72, No. 12
0099-2240/06/$08.00+0     doi:10.1128/AEM.01148-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Molecular Detection of Epiphytic Acaryochloris spp. on Marine Macroalgae{triangledown}

Satoshi Ohkubo,1 Hideaki Miyashita,1,2* Akio Murakami,3 Haruko Takeyama,4 Tohru Tsuchiya,1,2 and Mamoru Mimuro1,2

Graduate School of Human and Environmental Studies, Kyoto University, Kyoto 606-8501, Japan,1 Hall of Global Environmental Research, Kyoto University, Kyoto 606-8501, Japan,2 Kobe University Research Center for Inland Sea, Iwaya, Awaji, Hyogo 656-2401, Japan,3 Faculty of Engineering, Tokyo University of Technology and Agriculture, Koganei, Tokyo 184-8588, Japan4

Received 18 May 2006/ Accepted 26 September 2006

A molecular method for detecting the epiphyte community on marine macroalgae was developed by using PCR-denaturing gradient gel electrophoresis. Selective amplification of 16S rRNA gene fragments from either cyanobacteria or algal plastids improved the detection of minor epiphytes. Two phylotypes of Acaryochloris, a chlorophyll d-containing cyanobacterium, were found not only on red macroalgae but also on green and brown macroalgae.

* Corresponding author. Mailing address: Graduate School of Human and Environmental Studies, Kyoto University, Kyoto 606-8501, Japan. Phone: 81-75-753-7928. Fax: 81-75-753-7928. E-mail: miyashita{at}hm1.mbox.media.kyoto-u.ac.jp.

{triangledown} Published ahead of print on 6 October 2006.

Applied and Environmental Microbiology, December 2006, p. 7912-7915, Vol. 72, No. 12
0099-2240/06/$08.00+0     doi:10.1128/AEM.01148-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Swingley, W. D., Chen, M., Cheung, P. C., Conrad, A. L., Dejesa, L. C., Hao, J., Honchak, B. M., Karbach, L. E., Kurdoglu, A., Lahiri, S., Mastrian, S. D., Miyashita, H., Page, L., Ramakrishna, P., Satoh, S., Sattley, W. M., Shimada, Y., Taylor, H. L., Tomo, T., Tsuchiya, T., Wang, Z. T., Raymond, J., Mimuro, M., Blankenship, R. E., Touchman, J. W. (2008). Niche adaptation and genome expansion in the chlorophyll d-producing cyanobacterium Acaryochloris marina. Proc. Natl. Acad. Sci. USA 105: 2005-2010 [Abstract] [Full Text]  


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