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Applied and Environmental Microbiology, December 2006, p. 7925-7929, Vol. 72, No. 12
0099-2240/06/$08.00+0     doi:10.1128/AEM.01548-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Characterization of a Vibrio alginolyticus Strain, Isolated from Alaskan Oysters, Carrying a Hemolysin Gene Similar to the Thermostable Direct Hemolysin-Related Hemolysin Gene (trh) of Vibrio parahaemolyticus{triangledown}

Narjol González-Escalona,1,2* George M. Blackstone,2 and Angelo DePaola2

Department of Food Science, North Carolina State University, Raleigh, North Carolina,1 FDA Gulf Coast Seafood Laboratory, Dauphin Island, Alabama2

Received 5 July 2006/ Accepted 9 October 2006

A Vibrio strain isolated from Alaskan oysters and classified by its biochemical characteristics as Vibrio alginolyticus possessed a thermostable direct hemolysin-related hemolysin (trh) gene previously reported only in Vibrio parahaemolyticus. This trh-like gene was cloned and sequenced and was 98% identical to the trh2 gene of V. parahaemolyticus. This gene seems to be functional since it was transcriptionally active in early-stationary-phase growing cells. To our knowledge, this is the first report of V. alginolyticus possessing a trh gene.


* Corresponding author. Mailing address: FDA Gulf Coast Seafood Laboratory, 1 Iberville Dr., Dauphin Island, AL 36528. Phone: (251) 690-3074. Fax: (251) 694-4477. E-mail: narjol{at}gmail.com.

{triangledown} Published ahead of print on 20 October 2006.


Applied and Environmental Microbiology, December 2006, p. 7925-7929, Vol. 72, No. 12
0099-2240/06/$08.00+0     doi:10.1128/AEM.01548-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.




This article has been cited by other articles:

  • Nordstrom, J. L., Vickery, M. C. L., Blackstone, G. M., Murray, S. L., DePaola, A. (2007). Development of a Multiplex Real-Time PCR Assay with an Internal Amplification Control for the Detection of Total and Pathogenic Vibrio parahaemolyticus Bacteria in Oysters. Appl. Environ. Microbiol. 73: 5840-5847 [Abstract] [Full Text]