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Applied and Environmental Microbiology, December 2006, p. 7945-7948, Vol. 72, No. 12
0099-2240/06/$08.00+0     doi:10.1128/AEM.01052-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

Aerobic Growth of Escherichia coli with 2,4,6-Trinitrotoluene (TNT) as the Sole Nitrogen Source and Evidence of TNT Denitration by Whole Cells and Cell-Free Extracts{triangledown} ,{dagger}

Ben Stenuit,1 Laurent Eyers,1 Raoul Rozenberg,2 Jean-Louis Habib-Jiwan,2 and Spiros N. Agathos1*

Unit of Bioengineering, Department of Applied ChemistryBioindustries,1 Mass Spectrometry Unit, Department of Chemistry, Catholic University of Louvain, Louvain-la-Neuve, Belgium2

Received 5 May 2006/ Accepted 25 September 2006

Escherichia coli grew aerobically with 2,4,6-trinitrotoluene (TNT) as sole nitrogen source and caused TNT's partial denitration. This reaction was enhanced in nongrowing cell suspensions with 0.516 mol nitrite released per mol TNT. Cell extracts denitrated TNT in the presence of NAD(P)H. Isomers of amino-dimethyl-tetranitrobiphenyl were detected and confirmed with U-15N-labeled TNT.

* Corresponding author. Mailing address: Unit of Bioengineering, Place Croix du Sud 2/19, 1348 Louvain-la-Neuve, Belgium. Phone: 32 10 47 36 44. Fax: 32 10 47 30 62. E-mail: spiros.agathos{at}uclouvain.be.

{triangledown} Published ahead of print on 29 September 2006.

{dagger} Supplemental material for this article may be found at http://aem.asm.org/.

Applied and Environmental Microbiology, December 2006, p. 7945-7948, Vol. 72, No. 12
0099-2240/06/$08.00+0     doi:10.1128/AEM.01052-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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  • Ziganshin, A. M., Gerlach, R., Borch, T., Naumov, A. V., Naumova, R. P. (2007). Production of Eight Different Hydride Complexes and Nitrite Release from 2,4,6-Trinitrotoluene by Yarrowia lipolytica. Appl. Environ. Microbiol. 73: 7898-7905 [Abstract] [Full Text]  


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