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Applied and Environmental Microbiology, December 2006, p. 7949-7953, Vol. 72, No. 12
0099-2240/06/$08.00+0     doi:10.1128/AEM.01046-06

SHORT REPORT

Sequence Analysis of the rfb Loci, Encoding Proteins Involved in the Biosynthesis of the Salmonella enterica O17 and O18 Antigens: Serogroup-Specific Identification by PCR{triangledown}

Collette Fitzgerald,* Linda Gheesling, Marcus Collins, and Patricia I. Fields

Foodborne and Diarrheal Diseases Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

Received 5 May 2006/ Accepted 9 October 2006

We report sequencing of the O antigen encoded by the rfb gene cluster of Salmonella enterica serotype Jangwani (O17) and Salmonella serotype Cerro (O18). We developed serogroup O17- and O18-specific PCR assays based on rfb gene targets and found them to be sensitive and specific for rapid identification of Salmonella serogroups O17 and O18.

* Corresponding author. Mailing address: Foodborne and Diarrheal Diseases Branch, Division of Bacterial and Mycotic Diseases, NCID, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA 30333. Phone: (404) 639-0838. Fax: (404) 639-3333. E-mail: chf3{at}cdc.gov.

{triangledown} Published ahead of print on 20 October 2006.

Applied and Environmental Microbiology, December 2006, p. 7949-7953, Vol. 72, No. 12
0099-2240/06/$08.00+0     doi:10.1128/AEM.01046-06




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