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Applied and Environmental Microbiology, December 2006, p. 7959-7961, Vol. 72, No. 12
0099-2240/06/$08.00+0     doi:10.1128/AEM.01857-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

SHORT REPORT

In Vitro Deoxynucleotidylation of the Terminal Protein of Streptomyces Linear Chromosomes{triangledown}

Chien-Chin Yang,1 Yi-Hong Chen,1 Hsiu-Hui Tsai,2 Chih-Hung Huang,2,3 Tzu-Wen Huang,2 and Carton W. Chen2*

Department of Chemistry, Chung-Yuan Christian University, Chung-li, Taiwan,1 Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Shih-Pai,2 Institute of Biotechnology, National Taipei University of Technology, Taipei, Taiwan3

Received 4 August 2006/ Accepted 10 October 2006

Single-stranded gaps at the 3' ends of Streptomyces linear replicons are patched by DNA synthesis primed by terminal proteins (TP) during replication. We devised an in vitro system that specifically incorporated dCMP, the first nucleotide at the 5' ends, onto a threonine residue of the TP of Streptomyces coelicolor.

* Corresponding author. Mailing address: Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Shih-Pai, Taipei, Taiwan. Phone: 886-2 2826 7040. Fax: 886-2 2826 4930. E-mail: cwchen{at}ym.edu.tw.

{triangledown} Published ahead of print on 20 October 2006.

Applied and Environmental Microbiology, December 2006, p. 7959-7961, Vol. 72, No. 12
0099-2240/06/$08.00+0     doi:10.1128/AEM.01857-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lin, Y.-r., Hahn, M.-Y., Roe, J.-H., Huang, T.-W., Tsai, H.-H., Lin, Y.-F., Su, T.-S., Chan, Y.-J., Chen, C. W. (2009). Streptomyces Telomeres Contain a Promoter. J. Bacteriol. 191: 773-781 [Abstract] [Full Text]  
  • Tsai, H.-H., Huang, C.-H., Lin, A. M., Chen, C. W. (2008). Terminal proteins of Streptomyces chromosome can target DNA into eukaryotic nuclei. Nucleic Acids Res 36: e62-e62 [Abstract] [Full Text]  
  • Huang, T.-W., Chen, C. W. (2008). DNA Polymerase I Is Not Required for Replication of Linear Chromosomes in Streptomyces. J. Bacteriol. 190: 755-758 [Abstract] [Full Text]  
  • Parschat, K., Overhage, J., Strittmatter, A. W., Henne, A., Gottschalk, G., Fetzner, S. (2007). Complete Nucleotide Sequence of the 113-Kilobase Linear Catabolic Plasmid pAL1 of Arthrobacter nitroguajacolicus Ru61a and Transcriptional Analysis of Genes Involved in Quinaldine Degradation. J. Bacteriol. 189: 3855-3867 [Abstract] [Full Text]  


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