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Applied and Environmental Microbiology, February 2006, p. 1079-1085, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1079-1085.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Pure-Culture Growth of Fermentative Bacteria, Facilitated by H2 Removal: Bioenergetics and H2 Production

Cameron J. Adams,1,{dagger} Molly C. Redmond,2 and David L. Valentine3*

Marine Biology Research Division, Scripps Institution of Oceanography, and Department of Chemistry and Biochemistry, University of California, San Diego,1 Graduate Program in Marine Science, University of California, Santa Barbara,2 Department of Earth Science and Marine Science Institute, University of California, Santa Barbara, California3

Received 12 August 2005/ Accepted 8 November 2005

We used an H2-purging culture vessel to replace an H2-consuming syntrophic partner, allowing the growth of pure cultures of Syntrophothermus lipocalidus on butyrate and Aminobacterium colombiense on alanine. By decoupling the syntrophic association, it was possible to manipulate and monitor the single organism's growth environment and determine the change in Gibbs free energy yield ({Delta}G) in response to changes in the concentrations of reactants and products, the purging rate, and the temperature. In each of these situations, H2 production changed such that {Delta}G remained nearly constant for each organism (–11.1 ± 1.4 kJ mol butyrate–1 for S. lipocalidus and –58.2 ± 1.0 kJ mol alanine–1 for A. colombiense). The cellular maintenance energy, determined from the {Delta}G value and the hydrogen production rate at the point where the cell number was constant, was 4.6 x 10–13 kJ cell–1 day–1 for S. lipocalidus at 55°C and 6.2 x 10–13 kJ cell–1 day–1 for A. colombiense at 37°C. S. lipocalidus, in particular, seems adapted to thrive under conditions of low energy availability.

* Corresponding author. Mailing address: Department of Earth Science and Marine Science Institute, University of California, Santa Barbara, CA 93106. Phone: (805) 893-2973. Fax: (805) 893-2314. E-mail: valentine{at}geol.ucsb.edu.

{dagger} Present address: Invitrogen Corporation, 1600 Faraday Avenue, Carlsbad, CA 92008.

Applied and Environmental Microbiology, February 2006, p. 1079-1085, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1079-1085.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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