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Applied and Environmental Microbiology, February 2006, p. 1135-1140, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1135-1140.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Biocontrol of the Food-Borne Pathogens Listeria monocytogenes and Salmonella enterica Serovar Poona on Fresh-Cut Apples with Naturally Occurring Bacterial and Yeast Antagonists

Britta Leverentz,¹ William S. Conway,¹ Wojciech Janisiewicz,^2* Maribel Abadias,³ Cletus P. Kurtzman,⁴ and Mary J. Camp⁵

Produce Quality and Safety Laboratory,¹ Biometrical Consulting Service, Henry A. Wallace Beltsville Agricultural Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705,⁴ Appalachian Fruit Research Station, Agricultural Research Service, U.S. Department of Agriculture, Kearneysville, West Virginia 25430,² Institut de Recerca i Tecnologia Agroalimentaries, Centre R&D, 25198 Lleida Catalonia, Spain,³ Microbial Genomics and Bioprocessing Research Unit, National Center for Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604⁵

Received 15 June 2005/ Accepted 19 November 2005

Fresh-cut apples contaminated with either Listeria monocytogenes or Salmonella enterica serovar Poona, using strains implicated in outbreaks, were treated with one of 17 antagonists originally selected for their ability to inhibit fungal postharvest decay on fruit. While most of the antagonists increased the growth of the food-borne pathogens, four of them, including Gluconobacter asaii (T1-D1), a Candida sp. (T4-E4), Discosphaerina fagi (ST1-C9), and Metschnikowia pulcherrima (T1-E2), proved effective in preventing the growth or survival of food-borne human pathogens on fresh-cut apple tissue. The contaminated apple tissue plugs were stored for up to 7 days at two different temperatures. The four antagonists survived or grew on the apple tissue at 10 or 25°C. These four antagonists reduced the Listeria monocytogenes populations and except for the Candida sp. (T4-E4), also reduced the S. enterica serovar Poona populations. The reduction was higher at 25°C than at 10°C, and the growth of the antagonists, as well as pathogens, increased at the higher temperature.

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* Corresponding author. Mailing address: USDA-ARS, Appalachian Fruit Research Station, 2217 Wiltshire Rd., Kearneysville, WV 25430. Phone: (304) 725-3451. Fax: (304) 728-2340. E-mail: wjanisiewicz{at}afrs.ars.usda.gov.

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Applied and Environmental Microbiology, February 2006, p. 1135-1140, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1135-1140.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

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