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Applied and Environmental Microbiology, February 2006, p. 1148-1156, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1148-1156.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Molecular and Genetic Characterization of a Novel Nisin Variant Produced by Streptococcus uberis

Ruth E. Wirawan, Nikolai A. Klesse, Ralph W. Jack, and John R. Tagg*

Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand

Received 22 September 2005/ Accepted 14 November 2005

Streptococcus uberis is one of the principal causative agents of bovine mastitis. In this study, we report that S. uberis strain 42 produces a lantibiotic, nisin U, which is 78% identical (82% similar) to nisin A from Lactococcus lactis. The 15.6-kb nisin U locus comprises 11 open reading frames, similar in putative functionality but differing in arrangement from that of the nisin A biosynthetic cluster. The nisin U producer strain exhibits specific resistance (immunity) to nisin U and cross-resistance to nisin A, a finding consistent with the 55% sequence similarity of their respective immunity peptides. Homologues of the nisin U structural gene were identified in several additional S. uberis strains, and in each case cross-protective immunity was expressed to nisin A and to the other producers of nisin U and its variants. To our knowledge, this is the first report both of characterization of a bacteriocin by S. uberis, as well as of a member of the nisin family of peptides in a species other than L. lactis.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Otago, P.O. Box 56, Dunedin, New Zealand. Phone: 64-3-479-7714. Fax: 64-3-479-8540. E-mail: john.tagg{at}stonebow.otago.ac.nz.

Applied and Environmental Microbiology, February 2006, p. 1148-1156, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1148-1156.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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