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Applied and Environmental Microbiology, February 2006, p. 1148-1156, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1148-1156.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Molecular and Genetic Characterization of a Novel Nisin Variant Produced by Streptococcus uberis

Ruth E. Wirawan, Nikolai A. Klesse, Ralph W. Jack, and John R. Tagg*

Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand

Received 22 September 2005/ Accepted 14 November 2005

Streptococcus uberis is one of the principal causative agents of bovine mastitis. In this study, we report that S. uberis strain 42 produces a lantibiotic, nisin U, which is 78% identical (82% similar) to nisin A from Lactococcus lactis. The 15.6-kb nisin U locus comprises 11 open reading frames, similar in putative functionality but differing in arrangement from that of the nisin A biosynthetic cluster. The nisin U producer strain exhibits specific resistance (immunity) to nisin U and cross-resistance to nisin A, a finding consistent with the 55% sequence similarity of their respective immunity peptides. Homologues of the nisin U structural gene were identified in several additional S. uberis strains, and in each case cross-protective immunity was expressed to nisin A and to the other producers of nisin U and its variants. To our knowledge, this is the first report both of characterization of a bacteriocin by S. uberis, as well as of a member of the nisin family of peptides in a species other than L. lactis.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Otago, P.O. Box 56, Dunedin, New Zealand. Phone: 64-3-479-7714. Fax: 64-3-479-8540. E-mail: john.tagg{at}stonebow.otago.ac.nz.

Applied and Environmental Microbiology, February 2006, p. 1148-1156, Vol. 72, No. 2
0099-2240/06/$08.00+0     doi:10.1128/AEM.72.2.1148-1156.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Heng, N. C. K., Burtenshaw, G. A., Jack, R. W., Tagg, J. R. (2007). Ubericin A, a Class IIa Bacteriocin Produced by Streptococcus uberis. Appl. Environ. Microbiol. 73: 7763-7766 [Abstract] [Full Text]  
  • Rink, R., Wierenga, J., Kuipers, A., Kluskens, L. D., Driessen, A. J. M., Kuipers, O. P., Moll, G. N. (2007). Dissection and Modulation of the Four Distinct Activities of Nisin by Mutagenesis of Rings A and B and by C-Terminal Truncation. Appl. Environ. Microbiol. 73: 5809-5816 [Abstract] [Full Text]  
  • Wirawan, R. E., Swanson, K. M., Kleffmann, T., Jack, R. W., Tagg, J. R. (2007). Uberolysin: a novel cyclic bacteriocin produced by Streptococcus uberis. Microbiology 153: 1619-1630 [Abstract] [Full Text]  
  • Robson, C. L., Wescombe, P. A., Klesse, N. A., Tagg, J. R. (2007). Isolation and partial characterization of the Streptococcus mutans type AII lantibiotic mutacin K8. Microbiology 153: 1631-1641 [Abstract] [Full Text]  
  • Nes, I. F., Diep, D. B., Holo, H. (2007). Bacteriocin Diversity in Streptococcus and Enterococcus. J. Bacteriol. 189: 1189-1198 [Full Text]  
  • Takala, T. M., Saris, P. E. J. (2006). C terminus of NisI provides specificity to nisin. Microbiology 152: 3543-3549 [Abstract] [Full Text]  


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